USP48 蛋白, Human (Sf9, FLAG)

USP48 protein, a deubiquitinase, hydrolyzes ubiquitin bonds at the C-terminal Gly, processing poly-ubiquitin precursors and ubiquitinated proteins. It may regulate NF-kappa-B activation by the TNF receptor superfamily via RELA and TRAF2 interactions. With potential roles at postsynaptic sites, USP48 exhibits multifunctionality, influencing ubiquitin processing and signaling pathways involved in immune response and synaptic function. USP48 Protein, Human (Sf9, FLAG) is the recombinant human-derived USP48 protein, expressed by Sf9 insect cells , with C-Flag labeled tag. The total length of USP48 Protein, Human (Sf9, FLAG) is 1034 a.a., .

USP48 蛋白作为去泛素酶发挥作用，特异性识别并水解泛素 C 端 Gly 的肽键。它涉及多聚泛素前体和泛素化蛋白质的加工。此外，USP48 可能通过与 RELA 和 TRAF2 相互作用，在调节 TNF 受体超家族激活 NF-κ-B 中发挥作用。此外，建议在突触后位点具有潜在的调节功能。这些不同的活性凸显了 USP48 在细胞过程中的多功能性质，包括泛素加工和与免疫反应和突触功能有关的信号通路的调节。

The fundamental role of USP48 is specific removal of ubiquitin from substrates. USP48 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

C-Flag

Q86UV5 (A2-H1035)

84196

Approximately 120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.