1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Hydrolases (EC 3) Deubiquitinase
  4. Ubiquitin-Specific Protease
  5. Ubiquitin specific peptidase 51 (USP51)
  6. USP51 蛋白, Human (Sf9, His, FLAG)

USP51 蛋白, Human (Sf9, His, FLAG)

目录号: HY-P701425
产品使用指南

USP51 蛋白通过在双链断裂处使组蛋白 H2A 的“Lys-14”(H2AK13Ub) 和“Lys-16”(H2AK15Ub) 残基去泛素化来独特地调节 DNA 损伤反应。DNA 损伤后,USP51 被招募到染色质并调节 TP53BP1 和 BRCA1 组装/分解,这对于 DNA 修复至关重要。USP51 蛋白, Human (Sf9, His, FLAG) 是重组的 USP51 蛋白,由 Sf9 insect cells 表达,带有 N-Flag, N-8*His 标签。USP51 蛋白, Human (Sf9, His, FLAG) 全长 344 个氨基酸。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The USP51 protein uniquely regulates the DNA damage response by deubiquitinating the "Lys-14" (H2AK13Ub) and "Lys-16" (H2AK15Ub) residues of histone H2A at double-strand breaks. After DNA damage, USP51 is recruited to chromatin and regulates TP53BP1 and BRCA1 assembly/disassembly, which is critical for DNA repair. USP51 Protein, Human (Sf9, His, FLAG) is the recombinant human-derived USP51 protein, expressed by Sf9 insect cells , with N-Flag, N-8*His labeled tag. The total length of USP51 Protein, Human (Sf9, His, FLAG) is 344 a.a., .

研究背景

USP51 蛋白通过特异性去泛素化组蛋白 H2A 双链断裂 (DSB) 处的“Lys-14”(H2AK13Ub) 和“Lys-16”(H2AK15Ub) 残基,在 DNA 损伤反应的调节中发挥独特作用。DNA 损伤后,USP51 被招募到染色质,在那里它主动调节 TP53BP1 和 BRCA1(DNA 修复过程中的关键角色)的动态组装和分解。除了在组蛋白 H2A 去泛素化中发挥作用外,USP51 还表现出对“Lys-27”或“Lys-63”连接的双泛素的活性,进一步扩大了其对泛素信号通路的影响。USP51 在这些分子过程中的复杂参与强调了它在协调 DNA 损伤反应中的重要性,并强调了它作为维持基因组完整性的关键参与者的潜力。

种属

Human

表达系统

Sf9 insect cells

标签

N-Flag;N-8*His

蛋白编号

Q70EK9 (R363-Q706)

基因 ID

158880

蛋白结构
N-term
8*His-Flag
USP51 (R363-Q706)
Accession # Q70EK9
C-term
同用名
USP51; Ubiquitin carboxyl-terminal hydrolase 51; Deubiquitinating enzyme 51; Ubiquitin thioesterase 51; Ubiquitin-specific-processing protease 51
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
USP51 Protein, Human (Sf9, His, FLAG)
目录号:
HY-P701425
需求量: