USP6 蛋白, Human (Sf9, GST)

USP6 protein is an ATP-independent isopeptidase with unique deubiquitinase activity that can cleave the C-terminus of ubiquitin and catalyze its own deubiquitination. Notably, it coordinates ARF6 plasma membrane localization and selectively affects ARF6-dependent endocytic protein trafficking. USP6 Protein, Human (Sf9, GST) is the recombinant human-derived USP6 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of USP6 Protein, Human (Sf9, GST) is 878 a.a., .

USP6 是一种不依赖于 ATP 的异肽酶，具有独特的去泛素酶活性，在泛素部分的 C 末端裂解并催化其自身去泛素化。在体外，与异构体 3 相比，异构体 2 表现出去泛素化活性。值得注意的是，USP6 在协调 ARF6 的质膜定位和选择性调节 ARF6 依赖性内吞蛋白运输方面发挥着至关重要的作用。其功能意义延伸至肿瘤发生起始，因为它通过 NF-kappa-B 激活诱导基质金属蛋白酶的产生。USP6 的多方面活性凸显了其在细胞过程中的关键作用，使其成为蛋白质调节、膜动力学和肿瘤发生中涉及的复杂平衡的关键参与者。

The fundamental role of USP6 is specific removal of ubiquitin from substrates. USP6 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader. At a concentration of 0.03 nM, USP6 reacts with the Ub-Rho110 substrate. The signal window after half an hour of reaction can be greater than or equal to 10.

Human

Sf9 insect cells

N-GST

P35125 (K529-Q1406)

9098

Approximately 125.1 KDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.