VCAM-1/CD106 蛋白, Human (HEK293, Fc)

VCAM-1/CD106 proteins expressed on endothelial cells critically regulate leukocyte adhesion through integrin interactions, initiating calcium channel activation and RAC1-mediated transendothelial migration during inflammation. VCAM-1/CD106 Protein, Human (HEK293, Fc) is the recombinant human-derived VCAM-1/CD106 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of VCAM-1/CD106 Protein, Human (HEK293, Fc) is 673 a.a., with molecular weight of 110-150 kDa.

VCAM-1/CD106 是一种主要在内皮细胞上表达的细胞粘附糖蛋白，在免疫监视和炎症中发挥着关键作用。作为白细胞粘附到内皮的主要调节因子，它与各种整合素相互作用，影响免疫细胞的相互作用。在炎症反应期间，VCAM-1 与激活的内皮细胞上的配体结合，启动钙通道激活和 RAC1 小 GTP 酶激活，促进白细胞跨内皮迁移。此外，VCAM-1 充当进入骨髓的质量控制检查点，在主要组织相容性复合体 (MHC) I 类呈现的背景下提供“不要吃我”信号。

Measured by the ability of the proliferation protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ this effect is 0.1999 ng/ml, corresponding to a specific activity is 5.003×10⁶ units/mg.

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  Measured by the ability of the proliferation protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ this effect is 0.1999 ng/mL, corresponding to a specific activity is 5.003×10⁶ units/mg.

Human

HEK293

C-hFc

P19320 (F25-P697)

7412  [NCBI]

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSP

110-150 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.