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  3. Hydrolases (EC 3)
  4. VCPIP1 蛋白, Human (Sf9)

VCPIP1 蛋白, Human (Sf9)

目录号: HY-P701436
产品使用指南

VCPIP1 蛋白是一种多功能去泛素化酶,在多种细胞过程中发挥着至关重要的作用。它是 DNA 修复以及有丝分裂后高尔基体和内质网重新组装的重要组成部分,促进 VCP 介导的高尔基体堆积和 tER 形成。VCPIP1 蛋白, Human (Sf9) 是重组的 VCPIP1 蛋白,由 Sf9 insect cells 表达,不带标签。VCPIP1 蛋白, Human (Sf9) 全长 1222 个氨基酸。

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描述

The VCPIP1 protein is a multifunctional deubiquitinating enzyme that plays a crucial role in a variety of cellular processes. It is an integral component of DNA repair and postmitotic Golgi and endoplasmic reticulum reassembly, promoting VCP-mediated Golgi stacking and tER formation. VCPIP1 Protein, Human (Sf9) is the recombinant human-derived VCPIP1 protein, expressed by Sf9 insect cells , with tag free. The total length of VCPIP1 Protein, Human (Sf9) is 1222 a.a., .

研究背景

VCPIP1 蛋白是一种多方面的去泛素化酶,在多种细胞过程中发挥着重要作用。它复杂地参与 DNA 修复以及有丝分裂后高尔基体和内质网的重新组装,促进 VCP 介导的高尔基体堆栈重新组装和过渡内质网 (tER) 的形成。此外,VCPIP1 参与包含 STX5A、NSFL1C 和 VCP 的三元复合物的解离。此外,在 ATMATR 磷酸化后,VCPIP1 通过催化 SPRTN 去泛素化、促进 SPRTN 募集到染色质并促进共价 DNA-蛋白质交联 (DPC) 的蛋白水解裂解来促进 DNA 修复。该酶通过水解“Lys-11”和“Lys-48”连接的多聚泛素链进一步证明了其多功能性。在微生物感染的情况下,VCPIP1通过催化A型肉毒杆菌神经毒素轻链(LC)去泛素化来调节A型肉毒杆菌神经毒素(BoNT/A)中毒的持续时间,从而防止LC被蛋白酶体降解并加速肉毒杆菌神经毒素中毒在患者中。这凸显了 VCPIP1 在细胞稳态和应对外部挑战中的多样化且关键的功能。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q96JH7 (M1-S1222)

基因 ID

80124

蛋白结构
N-term
VCPIP1 (M1-S1222)
Accession # Q96JH7
C-term
同用名
VCPIP1; Deubiquitinating protein VCPIP1; Valosin-containing protein p97/p47 complex-interacting protein 1; Valosin-containing protein p97/p47 complex-interacting protein p135; VCP/p47 complex-interacting 135-kDa protein
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

VCPIP1 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P701436
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