VE-Cadherin 蛋白, Rat (HEK293, His)

VE-cadherin is critical for cellular functions such as β-catenin binding and signaling receptor activity, and is involved in processes such as cell-cell junction organization and vascular maturation. It is predicted to localize in cell-cell junctions and nuclei, serving as a biomarker for pulmonary hypertension. VE-Cadherin Protein, Rat (HEK293, His) is the recombinant rat-derived VE-Cadherin protein, expressed by HEK293 , with C-His labeled tag. The total length of VE-Cadherin Protein, Rat (HEK293, His) is 561 a.a., with molecular weight of ~80-95 kDa.

VE-钙粘蛋白预计在各种细胞功能中发挥关键作用，包括 β-连环蛋白结合、酶结合和信号受体结合活性。预计它会参与细胞与细胞连接组织、蛋白质修饰调节和含蛋白质复合物组装调节等过程。VE-钙粘蛋白预计在血管成熟、细胞间粘附和细胞群增殖的负调节上游发挥作用。该蛋白预计位于细胞-细胞连接处、质膜外侧和细胞核中，并且可能参与连环蛋白复合物的形成。VE-钙粘蛋白具有预测的粘附连接和双细胞紧密连接活性，可作为肺动脉高压的生物标志物。其人类直系同源物钙粘蛋白 5 (CDH5) 在肺和心脏组织中表现出偏向表达，凸显了其在心血管和肺部环境中的重要性。

Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED₅₀ for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×10⁴ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED₅₀ for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×10⁴units/mg.

Rat

HEK293

C-His

NP_001100877 (G25-Q585)

307618  [NCBI]

GPNMLPVHQRQKRDWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEQAGKIFRVDADTGDVLAYERLDREKVSEYFLTALIVDKKTNKNLEQPSSFTVKVHDVNDNWPVFTHQVFNASVPEMSAIGTSVIRVTATDADDPTVAGHATVLYQMVKGNEYFAIDNSGLIFTKDKNLDRETRAEYKIVVEAQDAQGLRGESGTATVLIRLEDINDNFPIFTQSTYTFSVPEDIRVGKPLGSLSVEDPDEPQNRMTKYSIMQGEYRDTFTIETDPDRNEGIIKPTKPLDYELIQQYTFHIEATDPTIRLGYLSSTAGKNKAKIIINVLDVDEPPVFQRRFYHFHLPENKKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQYFRITKQGNIYNEKELDRETHAWYNLTVEANELDSRGNPVGKESIVQIYIEVLDENDNAPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNTKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHTKFHYLPVLISDNGMPSLTGTGTLTVAVCKCNEQGEFTFCEEMAAQ

Approximately 80-95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.