VEGF-CC 蛋白, Human (116a.a, HEK293)

VEGF-C Protein, Human (116a.a, HEK293) functions in lymphangiogenesis, where it acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth and migration.

VEGF-C 的主要功能是在淋巴管生成中，主要通过其受体 VEGFR-3 作用于淋巴管内皮细胞 (LEC)，促进存活、生长和迁移^[1]。然而，它除了对淋巴管有作用外，还可以促进血管的生长，调节其通透性。对血管的影响可通过其主要受体 VEGFR-3 或其次要受体 VEGFR-2^[2] 介导。除了血管靶标外，VEGF-C 对于神经发育和血压调节也很重要^[3][4]。

1.The ED₅₀ is <0.5 μg/mL as measured by HMVEC human microvascular endothelial cells.
2.Measured in a cell proliferation assay using HUVEC human microvascular endothelial cells. The ED₅₀ for this effect is ≤6.554 µg/mL, corresponding to a specific activity is ≥ 152.5 U/mg.

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  Measured in a cell proliferation assay using HUVEC human microvascular endothelial cells. The ED₅₀ for this effect is 6.554 µg/mL, corresponding to a specific activity is 152.5 U/mg.

Human

HEK293

Tag Free

P49767 (A112-R227)

7424  [NCBI]

AHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRR

16-19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Joukov V, et al. A novel vascular endothelial growth factor, VEGF-C, is a ligand for the Flt4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases. EMBO J. 1996 Jan 15;15(2):290-98.  [Content Brief]

  [2]. Tammela T, et al. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation Nature. 2008 Jul 31;454(7204):656-60.  [Content Brief]

  [3]. Le Bras B, et al. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Nat Neurosci. 2006 Mar;9(3):340-8.  [Content Brief]

  [4]. Machnik A, et al. Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism. Nat Med. 2009 May;15(5):545-52.  [Content Brief]