1. 重组蛋白
  2. Cytokines and Growth Factors
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-A
  6. VEGF121 蛋白, Human (HEK293)

VEGF121 蛋白, Human (HEK293)

目录号: HY-P73471
COA 产品使用指南

VEGF145 蛋白表达有限,表现出特殊的分布,并不广泛存在于组织中。它的有限出现意味着在生理过程中具有特定的、依赖于环境的作用。需要进一步的研究来揭示 VEGF145 积极参与的特定细胞环境和功能,从而深入了解其对局部生物活性的潜在贡献。VEGF121 蛋白, Human (HEK293) 是重组的 VEGF121 蛋白,由 HEK293 表达,不带标签。VEGF121 蛋白, Human (HEK293) 全长 147 个氨基酸,分子量分别约为 ~19.9 & 17.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1700 In-stock
20 μg ¥2850 In-stock
50 μg ¥4420 询价
100 μg ¥7500 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

VEGF145 Protein, with limited expression, exhibits specialized distribution and is not broadly present in tissues. Its restricted occurrence implies a specific, context-dependent role in physiological processes. Further research is needed to unveil the specific cellular contexts and functions where VEGF145 actively participates, providing insights into its potential contributions to localized biological activities. VEGF121 Protein, Human (HEK293) is the recombinant human-derived VEGF121 protein, expressed by HEK293 , with tag free. The total length of VEGF121 Protein, Human (HEK293) is 147 a.a., with molecular weight of ~19.9 & 17.0 kDa, respectively.

研究背景

VEGF145 蛋白的特点是表达有限,并未广泛分布于组织或细胞类型中。VEGF145 的有限出现表明其在生理过程中具有特殊且可能特定于环境的作用。需要进一步研究来揭示 VEGF145 积极参与的特定细胞环境和功能,从而揭示其对局部生物活性的潜在贡献。

生物活性

1.Immobilized human VEGF121 at 2μg/mL (100μL/well) can bind human VEGFR2-Fc and the EC50 is 5-50 ng/mL.
2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) and the ED50 is typically 3-15 ng/mL.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P15692-9 (M1-R147)

基因 ID
蛋白结构
N-term
VEGF121 (M1-R147)
Accession # P15692-9
C-term
同用名
VEGF-AA; MVCD1; VAS; Vascular endothelial growth factor A; VEGF; VPF
分子量

Approximately 19.9&17.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

VEGF121 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VEGF121 Protein, Human (HEK293)
目录号:
HY-P73471
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