VEGF164 蛋白, Mouse (164a.a, P.pastoris)

VEGF-A Protein is a key member of the VEGF family of cytokines.VEGF-A participates in angiogenesis, vasculogenesis, and endothelial cell growth, inducing endothelial cell proliferation, promoting cell migration, inhibiting cell apoptosis, and inducing vascular permeability.VEGF-A stimulates endothelial cell mitogenesis and cell migration.VEGF164 Protein, Mouse (P.pastoris) is the recombinant mouse-derived VEGF164 protein, expressed by P.pastoris , with tag free.

VEGF-A 是 VEGF 细胞因子家族的重要成员，其他成员还有 VEGF-B、-C、-D 和 PGF。VEGF-A 参与血管生成、血管发生和内皮细胞生长，诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡、诱导血管通透性。VEGF-A 与 FLT1/VEGFR1、KDR/VEGFR2 和 DEAR/FBXW7-AS1 受体、硫酸乙酰肝素和肝素结合。VEGF-A 还与 NRP1 结合，启动运动神经元轴突引导和细胞体迁移所需的信号通路，包括胚胎发育过程中面部运动神经元从菱形 4 到菱形 6 的尾部迁移。VEGF-A 刺激内皮细胞有丝分裂和细胞迁移。VEGF-A 也是一种血管扩张剂并增加微血管通透性^{[1][2][3][4][5]}。

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 3.54 ng/mL, corresponding to a specific activity is 2.82×10⁵ units/mg.

Mouse

P. pastoris

Tag Free

Q00731-2 (A27-R190)

22339  [NCBI]

APTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Approximately 18-22 kDa in reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.