VEGF165 蛋白, Human (P.pastoris)

VEGF165 Protein, Human (P.pastoris) is a Vascular Endothelial Growth Factor A (VEGF-A) isoform. VEGF is a heparin-binding growth factor specific for vascular endothelial cells that is able to induce angiogenesis.

血管内皮生长因子 (VEGF) 具有多种同工型，由选择性剪接或蛋白水解裂解产生，具有不同的受体结合和基质结合特性。已知这些亚型会产生一系列以分支差异为标志的血管生成模式，这对组织具有功能影响。VEGF-A 是 VEGF 细胞因子家族的关键成员，其他成员包括 VEGF-B、-C、-D 和 PlGF。VEGF-A 介导血管生成，即通过新血管的萌芽来扩张现有的血管床。vegfa 基因被翻译成多种剪接亚型，其中在人类中最引人注目的是 VEGF121、VEGF165 和 VEGF189^[1]。

The ED₅₀ is 1-5 ng/mL as measured by HUVEC cells, corresponding to a specific activity of 2 × 10⁵ - 1 × 10⁶ units/mg.

Human

P. pastoris

Tag Free

P15692-4 (A27-R191)

7422  [NCBI]

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Approximately 38.2 kDa (Disulfide-linked homodimer)

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.

<0.5 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Vempati P, et al. Extracellular regulation of VEGF: isoforms, proteolysis, and vascular patterning. Cytokine Growth Factor Rev. 2014 Feb;25(1):1-19.  [Content Brief]