VEGF164 蛋白, Rat (CHO)

VEGF164 Protein, Rat (CHO) is a potent mediator of angiogenesis and binds the semaphorin receptor, neuropilin1.

血管内皮生长因子（VEGF 或 VEGFA）是胎儿和成人血管生成和血管发生的有效介质。它是 PDGF 家族的一员，其特征是存在八个保守的半胱氨酸残基和一个胱氨酸结结构^[1]。VEGF164 似乎是最丰富、最有效的异构体，其次是 VEGF121 和 VEGF189^[1][2]。VEGF164 结合信号蛋白受体 Neuropilin1，并促进与 VEGF R2^[3] 形成复合物。

The ED₅₀ is <4 ng/mL as measured by HUVEC cells, corresponding to a specific activity of >2.5 × 10⁵ units/mg.

• 
  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 3.56 ng/mL, corresponding to a specific activity is 2.81×10⁵ units/mg.

Rat

CHO

Tag Free

P16612-2 (A27-R190)

83785  [NCBI]

APTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Approximately 35-48 kDa (Disulfide-linked homodimer) as determined by non-reducing SDS-PAGE; approximately 20-25 kDa as determined by reducing SDS-PAGE.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Robinson CJ, et al. The splice variants of vascular endothelial growth factor (VEGF) and their receptors. J Cell Sci. 2001 Mar;114(Pt 5):853-65.  [Content Brief]

  [2]. Byrne AM, et al. Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J Cell Mol Med. 2005 Oct-Dec;9(4):777-94.  [Content Brief]

  [3]. Qi Pan, et al. Neuropilin-1 Binds to VEGF121 and Regulates Endothelial Cell Migration and Sprouting. The Journal of Biological Chemistry 282, 24049-24056.