Vinculin 蛋白, Human

Vinculin Protein is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. It regulates adhesion by directly binding to actin, stimulating actin polymerization and recruiting actin remodeling proteins. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex, may also play important roles in cell morphology and locomotion. Vinculin Protein, Human is the recombinant human-derived Vinculin protein, expressed by E. coli , with tag free. The total length of Vinculin Protein, Human is 1065 a.a., with molecular weight of ~115.0 kDa.

Vinculin 是一种与细胞-细胞和细胞-基质连接相关的细胞骨架蛋白，被认为是参与将 F-肌动蛋白锚定到膜上的几种相互作用蛋白之一。Vinculin没有酶活性。它通过直接结合肌动蛋白、刺激肌动蛋白聚合和募集肌动蛋白重塑蛋白来调节粘附。在缺乏 Vinculin 的情况下，细胞-基质和细胞-细胞粘附显着受损，表明 Vinculin 在人体生理学中发挥着关键作用。Vinculin 可稳定粘着斑，从而抑制细胞迁移，这种效应可以通过肌醇磷脂局部浓度的短暂变化来缓解。Vinculin 提供机械连接，控制细胞信号传导过程，影响收缩性和粘附蛋白周转。Vinculin 被认为可以通过支持贴壁依赖性细胞生长和通过降低细胞运动来抑制肿瘤转移来发挥肿瘤抑制剂的作用。此外，Vinculin 调节细胞表面 E-cadherin 表达并增强 E-cadherin 复合物的机械感应，也可能在细胞形态和运动中发挥重要作用^[1][2][3][4]。

Human

E. coli

Tag Free

AAH39174.1 (P2-Q1066)

7414  [NCBI]

PVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTLEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Approximately 115.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.