VISTA/B7-H5 蛋白, Rat (HEK293, Fc)

VISTA/B7-H5 protein, an immunoregulatory receptor, inhibits T-cell response and may regulate embryonic stem cell differentiation by inhibiting BMP4 signaling. It also stimulates MMP14-mediated MMP2 activation, suggesting a role in matrix metalloproteinase processes. These roles highlight the significance of VISTA/B7-H5 in immune regulation, embryonic development, and extracellular matrix dynamics. VISTA/B7-H5 Protein, Rat (HEK293, Fc) is the recombinant rat-derived VISTA/B7-H5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of VISTA/B7-H5 Protein, Rat (HEK293, Fc) is 159 a.a., with molecular weight of ~44.7 kDa.

多项研究表明，VISTA/B7-H5 蛋白作为免疫调节受体，在抑制 T 细胞反应中发挥着关键作用。此外，它可能通过抑制 BMP4 信号传导促进胚胎干细胞的分化，展示了其在发育过程中的潜在作用。此外，VISTA/B7-H5 参与刺激 MMP14 介导的 MMP2 激活，表明在基质金属蛋白酶介导的过程中具有调节功能。这种多方面的作用强调了 VISTA/B7-H5 在免疫调节、胚胎发育和细胞外基质动力学中的重要性，揭示了其在不同生物环境中的潜在影响。

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in Jurkat human T lymphocytes. The ED₅₀ for this effect is 1.173 μg/mL in the presence of 5 μg/mL anti-CD3, corresponding to a specific activity is 852.515 U/mg.

Rat

HEK293

C-hFc

G3V9P3 (I33-A191)

690899  [NCBI]

IKVTTPYSLYVCPEGQNVTLTCRILDSVSKGHDANFLKTWFLSSRGEVQVCKEHRPIRNFISHHQHHRSHPAVNASHDQPQKHGLEIAYDNHGNFSITLHNVTLSDSGLYCCLVIEVKHHHPERRLYGYMELQVQTGKGSASTCTAYPPNEQDSDSITA

Approximately 60-75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.