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  3. VMO1 蛋白, Human (HEK293)

VMO1 蛋白, Human (HEK293)

目录号: HY-P77280
COA 产品使用指南

卵黄膜外层蛋白 1 同源物 (VMO1) 首次在鸡蛋卵黄膜外层被发现,与溶菌酶、VMO2 和卵黄蛋白一起存在。VMO1 是一种分泌蛋白,在内耳和泪膜中发挥重要作用,且可能与糖基化蛋白相互作用。VMO1 蛋白, Human (HEK293) 是重组的 VMO1 蛋白,由 HEK293 表达,不带标签。

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描述

Vitelline membrane outer layer protein 1 homolog (VMO1) is first characterized in the outer layer of the vitelline membrane of hen’s eggs, where VMO1is present together with lysozyme, VMO2, and ovomucin. VMO1 is a secreted protein and exerts important functions in inner ear and tear film, VMO1 may also interact with glycosylated proteins. VMO1 Protein, Human (HEK293) is the recombinant human-derived VMO1 protein, expressed by HEK293 , with tag free.

研究背景

卵黄膜外层蛋白 1 同源物 (VMO1) 首次在鸡蛋卵黄膜外层被发现,与溶菌酶、VMO2 和卵黄蛋白一起存在。对 VMO1 晶体结构的测定表明,VMO1 可能与糖基化蛋白相互作用。VMO1 是一种分泌蛋白,在内耳和泪膜中发挥重要作用[1]

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

Q7Z5L0-1 (Q25-S202)

基因 ID
蛋白结构
N-term
VMO1 (Q25-S202)
Accession # Q7Z5L0
C-term
同用名
Vitelline membrane outer layer protein 1 homolog; VMO1
氨基酸序列

QTDGRNGYTAVIEVTSGGPWGDWAWPEMCPDGFFASGFSLKVEPPQGIPGDDTALNGIRLHCARGNVLGNTHVVESQSGSWGEWSEPLWCRGGAYLVAFSLRVEAPTTLGDNTAANNVRFRCSDGEELQGPGLSWGDFGDWSDHCPKGACGLQTKIQGPRGLGDDTALNDARLFCCRS

分子量

Approximately 19.7 kDa.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

VMO1 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VMO1 Protein, Human (HEK293)
目录号:
HY-P77280
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