VSIG8 蛋白, Human (HEK293, His)

V-set and immunoglobulin domain-containing protein 8 (VSIG8) is a membrane protein belonging to complement receptor of the immunoglobulin superfamily. VSIG8 has RNA binding activity and is a human T-cell co-inhibitory ligand. VSIG-8 inhibits the production of cytokines, chemokines and other proteins on T cells, and also suppresses T cell proliferation and differentiation of na茂ve T cells. VSIG8 Protein, Human (HEK293, His) is the recombinant human-derived VSIG8 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of VSIG8 Protein, Human (HEK293, His) is 242 a.a., with molecular weight of 28-35 kDa.

V-系含免疫球蛋白结构域蛋白 8 (VSIG8) 是属于免疫球蛋白超家族补体受体的一种膜蛋白。VSIG8 具有 RNA 结合活性，且是人 T 细胞的共抑制配体。VSIG-8 抑制抗 CD3 激活的人 CD3 T 细胞产生细胞因子 (IL-2、IFN-γ、IL-17、IL-6和IL-19)、趋化因子 (MCP-1、MCP-10和IP-10) 和其他蛋白 (IGFBP3和RBP4)。在存在 T 细胞受体信号的情况下，VSIG-8 显著降低 CD4 和 CD8 T 细胞上 IFN-γ 和 IL-2 的产生。VSIG-8 显著抑制抗 CD3 诱导的人 T 细胞增殖，并显著降低 naïve CD4⁺ T 细胞向 Th1 细胞的转化^[1][2]。

Human

HEK293

C-6*His

P0DPA2 (V22-G263)

391123  [NCBI]

VRINGDGQEVLYLAEGDNVRLGCPYVLDPEDYGPNGLDIEWMQVNSDPAHHRENVFLSYQDKRINHGSLPHLQQRVRFAASDPSQYDASINLMNLQVSDTATYECRVKKTTMATRKVIVTVQARPAVPMCWTEGHMTYGNDVVLKCYASGGSQPLSYKWAKISGHHYPYRAGSYTSQHSYHSELSYQESFHSSINQGLNNGDLVLKDISRADDGLYQCTVANNVGYSVCVVEVKVSDSRRIG

28-35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAc-HAc, 5% Trehalose, pH 4.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.