Wilms tumor protein/WT1 蛋白, Human (His-SUMO)

Wilms tumor protein (WT1) is a key transcription factor that critically regulates cell development and survival by binding to 5'-GCG(T/G)GGGCG-3'. It oversees target genes such as erythropoietin (EPO) and is critical for the development of the genitourinary system. Wilms tumor protein/WT1 Protein, Human (His-SUMO) is the recombinant human-derived Wilms tumor protein/WT1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Wilms tumor protein/WT1 Protein, Human (His-SUMO) is 449 a.a., with molecular weight of ~65.2 kDa.

肾母细胞瘤蛋白 (WT1) 是一种关键转录因子，通过识别并结合 DNA 序列 5'-GCG(T/G)GGGCG-3'，在细胞发育和生存中发挥着至关重要的作用。它调节多种靶基因的表达，包括促红细胞生成素（EPO），对于泌尿生殖系统的发育是不可或缺的。WT1在肿瘤发生中表现出双重作用，既充当肿瘤抑制基因又充当癌基因。其功能可能是异构体特异性的，缺乏KTS基序的异构体充当转录因子，而含有KTS基序的异构体可能结合mRNA并参与mRNA代谢或剪接。值得注意的是，异构体 1 对 DNA 的亲和力较低，但可以结合 RNA。WT1 形成同型二聚体并与 WTIP 相互作用，主动翻译多聚体、核糖核蛋白 (mRNP) 颗粒、HNRNPU、U2AF2、CITED2、ZNF224、WTAP、SRY、AMER1、RBM4 等，突出其在细胞过程中的多样化相互作用和调节作用。

Human

E. coli

N-SUMO;N-6*His

P19544 (M1-L449)

7490  [NCBI]

MGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Approximately 65.2kDa

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  Greater than 93% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.2 μm filtered solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.