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  3. YebF 蛋白, E.coli (Myc, His)

YebF 蛋白, E.coli (Myc, His)

目录号: HY-P71453
产品使用指南

YebF 蛋白以单体形式存在于溶液中,与膜周质面的 OmpF/OmpC 相互作用,表明在细菌膜相关过程中发挥作用。其单体状态强调独立结构,促使进一步研究其与细菌膜中 OmpF/OmpC 相互作用的分子机制和功能影响。YebF 蛋白, E.coli (Myc, His) 是重组的 YebF 蛋白,由 E. coli 表达,带有 N-His, C-Myc 标签。

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描述

YebF protein, existing as a monomer in solution, interacts with OmpF/OmpC at the periplasmic face of the membrane, indicating a role in bacterial membrane-related processes. Its monomeric state emphasizes independent structure, prompting further investigation into the molecular mechanisms and functional impact of its interactions with OmpF/OmpC in the bacterial membrane. YebF Protein, E.coli (Myc, His) is the recombinant E. coli-derived YebF protein, expressed by E. coli , with N-His, C-Myc labeled tag.

研究背景

YebF 蛋白在溶液中作为单体观察到,表明其单独的结构状态。此外,YebF 与 OmpF/OmpC 相互作用,特别是在膜的周质面上。这些相互作用表明 YebF 在细菌膜中具有潜在作用,可能影响与膜动力学或运输相关的细胞过程。YebF 在溶液中的单体性质突出了其独立的结构形式,需要进一步探索以阐明其与细菌膜中 OmpF/OmpC 相互作用的具体分子机制和功能含义。

种属

E.coli

表达系统

E. coli

标签

N-His;C-Myc

蛋白编号

P33219 (A22-R118)

基因 ID

946363  [NCBI]

蛋白结构
N-term
10*His
YebF (A22-R118)
Accession # P33219
Myc
C-term
同用名
yebF; b1847; JW1836; Protein YebF
氨基酸序列

ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR

分子量

Approximately 15.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

YebF Protein, E.coli (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
YebF Protein, E.coli (Myc, His)
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HY-P71453
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