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  3. YTFE 蛋白, E.coli (GST)

YTFE 蛋白, E.coli (GST)

目录号: HY-P700386
产品使用指南

YTFE 蛋白是一种含二铁的分子,在氧化和亚硝化应激条件下至关重要地参与铁硫簇的修复。作为同型二聚体,YTFE 在减轻铁硫簇损伤方面发挥着关键作用,这是维持细胞内各种蛋白质完整性和功能所必需的过程。YTFE 蛋白, E.coli (GST) 是重组的 YTFE 蛋白,由 E. coli 表达,带有 N-GST 标签。YTFE 蛋白, E.coli (GST) 全长 220 个氨基酸,分子量为 51.9 kDa。

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描述

YTFE protein is a diiron-containing molecule that is crucially involved in the repair of iron-sulfur clusters under oxidative and nitrosative stress conditions. As a homodimer, YTFE plays a key role in mitigating damage to iron-sulfur clusters, a process necessary to maintain the integrity and function of various proteins within cells. YTFE Protein, E.coli (GST) is the recombinant E. coli-derived YTFE protein, expressed by E. coli , with N-GST labeled tag. The total length of YTFE Protein, E.coli (GST) is 220 a.a., with molecular weight of 51.9 kDa.

研究背景

YTFE 是一种含二铁的蛋白质,在氧化和亚硝化应激条件下容易受损的铁硫簇的修复中发挥着至关重要的作用。作为同二聚体,YTFE 参与细胞对环境压力的反应,这种反应可能会损害铁硫簇的完整性,而铁硫簇是各种生物过程中的关键辅助因子。YTFE 内的双铁中心可能有助于其作为修复酶的功能,促进受损铁硫簇的修复,并确保依赖于这些簇活性的蛋白质正常发挥功能(参考文献不可用)。

种属

E.coli

表达系统

E. coli

标签

N-GST

蛋白编号

P69506 (M1-E220)

基因 ID

66671871  [NCBI]

蛋白结构
N-term
GST
YTFE (M1-E220)
Accession # P69506
C-term
同用名
Iron-sulfur cluster repair protein YtfE; Regulator of cell morphogenesis and NO signaling; RCMNS
氨基酸序列

MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARKELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVERVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEHDEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALAGE

分子量

51.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

YTFE Protein, E.coli (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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