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  4. YUAB 蛋白, Bacillus subtilis (Cell-Free, His-SUMO)

YUAB 蛋白, Bacillus subtilis (Cell-Free, His-SUMO)

目录号: HY-P72009
产品使用指南

YUAB 是生物膜形成的重要贡献者，可自聚合形成具有优异稳定性和抗机械压缩性的疏水层。YUAB 对于复杂的集落结构至关重要，它与胞外多糖和 TasA 淀粉样原纤维协同相互作用，促进生物膜基质组装。YUAB 蛋白, Bacillus subtilis (Cell-Free, His-SUMO) 是重组的 YUAB 蛋白，由 E. coli Cell-free 表达，带有 N-His, N-SUMO 标签。YUAB 蛋白, Bacillus subtilis (Cell-Free, His-SUMO) 全长 181 个氨基酸，分子量约为 ~35.3 kDa。

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描述

YUAB is an important contributor to biofilm formation, self-polymerizing to form a hydrophobic layer with excellent stability and resistance to mechanical compression. YUAB is essential for complex colony structure, interacting synergistically with exopolysaccharides and TasA amyloid fibrils to facilitate biofilm matrix assembly. YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is the recombinant YUAB protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag. The total length of YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is 181 a.a., with molecular weight of ~35.3 kDa.
研究背景

YUAB 是生物膜形成的关键参与者，它参与自聚合，在生物膜表面形成独特的层，赋予疏水性。该层表现出稳定性和弹性，表现出对显着机械力压缩的抵抗力。YUAB 对于复杂集落结构的发展至关重要，它与胞外多糖和 TasA 淀粉样纤维的协同相互作用有关，促进生物膜基质的组装。作为聚合物形成实体，YUAB 有助于生物膜的结构完整性和疏水特性。
种属

Others
表达系统

E. coli Cell-free
标签

N-His;N-SUMO
蛋白编号

P71014 (M1-N181)
基因 ID

938831  [NCBI]
蛋白结构
N-term
6*His-SUMO
YUAB (M1-N181)
Accession # P71014
C-term
同用名
bslA; yuaB; BSU31080Biofilm-surface layer protein A; ORF-1
氨基酸序列

MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN

分子量

Approximately 35.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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