ZBP1 蛋白, Human (His)

ZBP1 is an important innate sensor that defends against viruses by recognizing Z-RNA structures and inducing PANoptosis. It binds Z-RNA through TNF-α family members and stimulates RIPK3 and MLKL to activate necroptosis. ZBP1 Protein, Human (His) is the recombinant human-derived ZBP1 protein, expressed by E. coli , with C-6*His labeled tag.

ZBP1 是一种关键的先天传感器，通过识别和结合疱疹病毒、正粘病毒和黄病毒等病原体产生的 Z-RNA 结构，在宿主防御各种病毒中发挥着至关重要的作用。这种识别触发一系列细胞死亡反应，包括焦亡、坏死性凋亡和细胞凋亡，统称为 PANoptosis。ZBP1 是坏死性凋亡的关键激活剂，特别是在响应诱导死亡的 TNF-α 家族成员时，通过结合 Z-RNA 并随后刺激 RIPK3 激酶，导致 MLKL 磷酸化和激活，最终执行程序性坏死。此外，在正粘病毒感染的情况下，ZBP1 检测受感染细胞核中的 Z-RNA 结构，激活 RIPK3 并促进 MLKL 磷酸化，导致核膜破坏并将细胞 DNA 释放到细胞质中。ZBP1 的作用超出了直接病原体感知的范围，因为它参与细菌和真菌感染引发的全凋亡。值得注意的是，为了应对寨卡病毒感染，ZBP1 与 RIPK3 结合，启动一种不依赖于死亡的转录程序，通过改变细胞代谢来限制病毒复制。然而，在单纯疱疹病毒 1 (HHV-1) 感染的情况下，ZBP1 可能与 HHV-1 蛋白 RIR1/ICP6 形成异质淀粉样蛋白结构，可能抑制 ZBP1 介导的坏死性凋亡，并允许病毒逃避宿主细胞死亡途径。

Human

E. coli

C-6*His

Q9H171-1 (M1-S149)

81030  [NCBI]

MAQAPADPGREGHLEQRILQVLTEAGSPVKLAQLVKECQAPKRELNQVLYRMKKELKVSLTSPATWCLGGTDPEGEGPAELALSSPAERPQQHAATIPETPGPQFSQQREEDIYRFLKDNGPQRALVIAQALGMRTAKDVNRDLYRMKS

16-23 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.