2. Apoptosis
  3. Apoptosis
  4. Sparfosic acid

Sparfosic acid 是 DNA 抗代谢剂和天门冬氨酸氨甲酰转移酶 (aspartate transcarbamoyl transferase) 的有效抑制剂。Sparfosic acid 具有抗肿瘤活性。天冬氨酸氨甲酰转移酶催化第二步 de novo 嘧啶生物合成。Sparfosic acid 协同增强 5-氟尿嘧啶 (5-FU) 和干扰素-α (IFN) 对人结肠癌细胞系的细胞毒性。

在相同的摩尔浓度下，化合物盐形式与游离形式有相同的生物活性，但盐形式 Sparfosic acid trisodium 通常具有更好的水溶性和稳定性。

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Sparfosic acid Chemical Structure

Sparfosic acid Chemical Structure

CAS No. : 51321-79-0

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Sparfosic acid 的其他形式现货产品:

Customer Review

Other Forms of Sparfosic acid:

Sparfosic acid 相关抗体

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MCE 顾客使用本产品发表的 2 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Sparfosic acid, a DNA antimetabolite agent, is a potent inhibitor of aspartate transcarbamoyl transferase, the enzyme catalyzing the second step of de novo pyrimidine biosynthesis. Sparfosic acid synergistically enhances the cytotoxicity of a combination of 5-fluorouracil (5-FU) and interferon-alpha (IFN) against human colon cancer cell lines[1][2][3].

体外研究
(In Vitro)

Sparfosic acid (N-(Phosphonacetyl)-L-aspartate, PALA) causes apoptosis in the resistant Br1 cells[1].
Sparfosic acid (300 µM) shows progressive accumulation of cells in S phase and activation of an apoptotic pathway leading to cell death[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Sparfosic acid 相关抗体:

Cell Viability Assay[1]

Cell Line: Br-l and L-2 cell lines established from metastasis in nude mouse injected with the human tumor cell line MDA-MB-435
Concentration: 300 µM
Incubation Time: 12, 24 and 48 hours
Result: Cells were predominantly in S phase in both the cell lines, although slightly higher proportion of cells in S phase were noted in L-2 than Brl-3prl cells.

Western Blot Analysis[1]

Cell Line: Br-l and L-2 cell lines
Concentration: 300 µM
Incubation Time: 4, 10 and 24 hours
Result: There was moderate difference in the level of phosphorylated Rb proteins seen in the two cell types. Marked increase in the amount of cyclin A protein was detected in the L-2 cells undergoing apoptosis with the highest level detected at 10 h post-drug treatment. In contrast, there was no increase in the level of cyclin A seen in the Brl-3prl cells. Cyclin E protein was found elevated in the L-2 cells and Brl-3prl cells compared to their respective controls.
体内研究
(In Vivo)

Sparfosic acid (490 mg/kg; i.p.; on days 1, 5, and 9; mice bearing B16 melanoma) shows the life-span is increased survives 77 to 86% longer than controls. Lewis lung carcinoma is highly sensitive to Sparfosic acid. Treatment on days 1, 5, and 9 following s.c. implantation of Lewis lung carcinoma is curative to 50% of the mice[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

255.12

Formula

C6H10NO8P
CAS 号
性状

液体（密度：1.792±0.06 g/cm3）

颜色

Colorless to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

H2O 中的溶解度 : 125 mg/mL (489.97 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.9197 mL 19.5986 mL 39.1972 mL
5 mM 0.7839 mL 3.9197 mL 7.8394 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: ≥98.0%

COA (262 KB)

产品使用指南 (1538 KB)
参考文献

Sparfosic acid 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.9197 mL 19.5986 mL 39.1972 mL 97.9931 mL
5 mM 0.7839 mL 3.9197 mL 7.8394 mL 19.5986 mL
10 mM 0.3920 mL 1.9599 mL 3.9197 mL 9.7993 mL
15 mM 0.2613 mL 1.3066 mL 2.6131 mL 6.5329 mL
20 mM 0.1960 mL 0.9799 mL 1.9599 mL 4.8997 mL
25 mM 0.1568 mL 0.7839 mL 1.5679 mL 3.9197 mL
30 mM 0.1307 mL 0.6533 mL 1.3066 mL 3.2664 mL
40 mM 0.0980 mL 0.4900 mL 0.9799 mL 2.4498 mL
50 mM 0.0784 mL 0.3920 mL 0.7839 mL 1.9599 mL
60 mM 0.0653 mL 0.3266 mL 0.6533 mL 1.6332 mL
80 mM 0.0490 mL 0.2450 mL 0.4900 mL 1.2249 mL
100 mM 0.0392 mL 0.1960 mL 0.3920 mL 0.9799 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sparfosic acid51321-79-0ApoptosisDNAantimetaboliteaspartatetranscarbamoyltransferasetumorpyrimidine biosynthesisInhibitorinhibitorinhibit

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