T4 UvsX Recombinase  (Synonyms: T4 UvsX 重组酶)

T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates^[1].

产品信息
T4 UvsX 重组酶来源于 T4 噬菌体，是 RecA/Rad51 家族的同源体，在双链 DN 断裂的修复和复制叉重新启动的过程中起到重要作用。采用 E.coli 重组表达，无核酸酶活性，储存液为 50 mM Tris-HCl, 50 mM KCI,1 mM DTT, 0.1 mM EDTA, 50% 甘油, pH 7.5。

使用说明
应用于等温扩增 RPA 反应，建议添加量为 300-400 ng/μL。

注意事项
避免反复冻融。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

液体

Colorless to light yellow

T4 UvsX 重组酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Morrical SW, et al. Amplification of snap-back DNA synthesis reactions by the uvsX recombinase of bacteriophage T4. J Biol Chem. 1991 Jul 25;266(21):14031-8.  [Content Brief]