Tetrabromorhodamine 123 bromide 

Tetrabromorhodamine 123 (TBR) bromide is a photosensitizer. Tetrabromorhodamine 123 bromide can be used for the research of photo dynamic therapy (PDT) and cancer^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
A. Labeling of Cells:
1. Incubate the cells according to your normal protocol.
2. Cells are incubated with DMEM containing 5 μM TBR and maintained in the dark in a CO₂ incubator at 37℃.
3. After 1 h, chang the medium to normal culture medium without phenol red, and cells were exposed to visible light with a 500 W Xe arc with a filter.
B. The intracellular localization of TBR:
1. Cells are cultured in 35 mm diameter glassbottomed dishes for 48 h. Incubate the cells according to your normal protocol.
2. For triple-staining, medium is changed to a solution containing BODIPY-TR ceramide (5 μM).
3. Cells are incubated at 48℃ for 20 min and then further incubated at 37℃ for 1 h, after which the solution is changed to normal medium containing 5 μM TBR.
4. The stock solution (1 mM) of Hoechst 33342 was added to the medium (final concentration, 100 μM), and cells were incubated for 15 min.
5. Cells are washed with phosphate-buffered saline (plus Ca²⁺, Mg²⁺) and then observed with the aid of confocal laser scanning microscopy (LSM).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

740.86

C₂₁H₁₃Br₅N₂O₃

623903-26-4

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Maiko Ogata, et al. Ca(2+)-dependent and caspase-3-independent apoptosis caused by damage in Golgi apparatus due to 2,4,5,7-tetrabromorhodamine 123 bromide-induced photodynamic effects. Photochem Photobiol. 2003 Sep;78(3):241-7.  [Content Brief]