UDP-Glc dehydrogenase (UGDH)  (Synonyms: UDP-葡萄糖脱氢酶（UGDH）)

UDP-Glc dehydrogenase (UGDH) catalyzes is a NAD+-dependent enzyme that catalyzes the two-fold oxidation of UDP-glucose (UDP-Glc) to produce UDP-glucuronic acid. UDP-Glc dehydrogenase (UGDH) is a key enzyme in the nucleotide-sugar interconversion pathway necessary for biosynthesis of many cell-wall polysaccharides^[1].

产品信息
本产品为重组大肠杆菌表达，C-terminal His Tagged，分子量 57 kDa，储存缓冲液为 20 mM Tris-HCl , pH 8.7，10% glycerol，主要用于从 UDP-Glc 生产 UDP-GlcA。

使用说明
1.按照反应体系加入底物，5 mM MgCl2，可适当加入缓冲盐 (如Tris-HCl) ，调节 pH 为 8.7，加入适量 UGDH (根据反应需求)；
2.混匀后，将样品置于 37℃ 孵育 2-4 h。

注意事项
体系放大时候，孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

液体

Colorless to light yellow

UDP-葡萄糖脱氢酶（UGDH）

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Jia T, et, al. UDP-Glucose Dehydrogenases: Identification, Expression, and Function Analyses in Upland Cotton (Gossypium hirsutum). Front Genet. 2021 Jan 11;11:597890.  [Content Brief]