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  3. Uniblue A sodium

Uniblue A sodium 是一种反应性蛋白质染料,可用于蛋白质的共价 pre-gel 染色 (Ex=594 nM)。

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Uniblue A sodium Chemical Structure

Uniblue A sodium Chemical Structure

CAS No. : 14541-90-3

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规格 价格 是否有货 数量
100 mg ¥720
3 - 4 周
1 g ¥2398
3 - 4 周
5 g   询价  
10 g   询价  

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM)[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare the derivatization buffer: 100 mM NaHCO3, 10% SDS, pH 8-9.
2. Add 10 μL of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 μL protein solution.
3. The samples are heated at 100°C for 1 min to perform the staining.
4. Add 100 μL of reducing solution composed of 10% glycerol and 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8.
5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis.
6. The sample is heated another minute at 100°C in order to achieve efficient reduction and allowed to cool to room temperature.
7. Add 20 μL alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes.
8. the samples are subjected to SDS-PAGE.
Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below).
As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

506.48

Formula

C22H15N2NaO7S2

CAS 号
Emission (Em)

594

Excitation (Ex)

555

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
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  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Uniblue A sodium
目录号:
HY-D1544
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