α1-3,4 Fucosidase, Bifidobacterium bifidum  (Synonyms: α1-3,4-岩藻糖苷酶)

α1-3,4 Fucosidase, Bifidobacterium bifidum is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1-3 and α1-4 linked fucose residues from oligosaccharides and glycoproteins^[1].

others

产品信息
本品利用大肠杆菌重组表达，C-terminal His-tag，分子量 160 kDa，缓冲液组分 20 mM Tris-HCl, pH 7.5, 500 mM NaCl ,10% glycerol，可催化水解低聚糖和糖蛋白末端 α1-3 和 α1-4 连接的岩藻糖残基。

使用说明
在反应体系中依次加入底物，50 mM 醋酸钠, pH 5.5，加入 5 mM CaCl2 混匀，加入适量酶。
将样品置于 37℃ 孵育 2-4h 或者过夜。

注意事项
体系放大时孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9037-65-4

液体

Colorless to light yellow

α1-3,4-岩藻糖苷酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Haruko Sakurama, et al. 1,3-1,4-α-l-Fucosynthase That Specifically Introduces Lewis a/x Antigens into Type-1/2 Chains. J Biol Chem. 2012 May 11; 287(20): 16709–16719.