α2,3-Sialyltransferase, Pasteurella multocida (P-1059)  (Synonyms: α2,3-唾液酸转移酶，来源多杀性巴氏杆菌 (P-1059); PmST1(M144D))

α2,3-Sialyltransferase, Pasteurella multocida (P-1059) (PmST1(M144D)), a sialyltransferase, can be isolated from Pasteurella multocida. alpha-2,3-Sialyltransferase (PmST1) can be converted into regioselective α2-6-sialyltransferase by saturation mutagenesis and regioselective screening^[1].

产品信息
该酶用于在非还原端 Gal 上以 α2,3 连接方式连接唾液酸。本品利用大肠杆菌重组表达，C-terminal His Tag，分子量 48.1 kDa，储存缓冲液组分 20 mM Tris-HCl，pH 8.0，10% glycerol。

使用方法
1. 按照反应体系加入底物，5 mM MgCl₂，可适当加入缓冲盐（如 Tris-HCl），调节 pH为 8.5，加入适量酶进行反应（根据反应需求）；
2. 混匀后,将样品置于 37℃ 孵育 1-2 h。

注意事项
体系放大时候，孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

71124-51-1

液体

Colorless to light yellow

α2,3-唾液酸转移酶，来源多杀性巴氏杆菌 (P-1059)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. McArthur JB, et al. Converting Pasteurella multocidaα2-3-sialyltransferase 1 (PmST1) to a regioselective α2-6-sialyltransferase by saturation mutagenesis and regioselective screening. Org Biomol Chem. 2017 Feb 21;15(7):1700-1709.  [Content Brief]