[Pyr1]-Apelin-13 (Synonyms: [pGlu1]-Apelin-13)

目录号: HY-P1033 纯度: 99.92%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

[Pyr1]-Apelin-13 是一种高效的，选择性的内源性 apelin 受体激动剂。

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Custom Peptide Synthesis

[Pyr1]-Apelin-13 Chemical Structure

CAS No. : 217082-60-5

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规格	价格	是否有货
1 mg	￥784	In-stock
2 mg	￥1254	In-stock
5 mg	￥2600	In-stock
10 mg	￥4200	In-stock
25 mg	现货	询价
50 mg		询价
100 mg		询价

or 大包装询价

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Customer Review

Other Forms of [Pyr1]-Apelin-13:

[Pyr1]-Apelin-13, Pro(¹³C₅,¹⁵N) TFA In-stock
[Pyr1]-Apelin-13, Gly(15N) TFA 询价

MCE 顾客使用本产品发表的 1 篇科研文献

•Research Square Print. 2023 Jan 23.

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生物活性
纯度 & 产品资料
参考文献

生物活性

[Pyr1]-Apelin-13 is a highly potent, selective endogenous apelin receptor (APJ) agonist.

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.0003 μM Compound: Pyr-Apelin-13	Agonist activity at human APJ receptor stably expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay Agonist activity at human APJ receptor stably expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay	[PMID: 31948845]
CHO	EC₅₀	0.002 μM Compound: Pyr-Apelin-13	Agonist activity at human APJ receptor stably expressed in CHO cells co-expressing Galphaq16 assessed as increase in intracellular calcium mobilization measured at 1 sec intervals for 90 secs by calcium 5 dye based FLIPR assay Agonist activity at human APJ receptor stably expressed in CHO cells co-expressing Galphaq16 assessed as increase in intracellular calcium mobilization measured at 1 sec intervals for 90 secs by calcium 5 dye based FLIPR assay	[PMID: 31948845]
CHO-K1	EC₅₀	0.001 μM Compound: Pyr-Apelin-13	Agonist activity at human APJ receptor stably expressed in CHOK1 cells assessed as induction of beta-arrestin 2 recruitment incubated for 90 mins by PathHunter assay Agonist activity at human APJ receptor stably expressed in CHOK1 cells assessed as induction of beta-arrestin 2 recruitment incubated for 90 mins by PathHunter assay	[PMID: 31948845]
HEK293	EC₅₀	0.047 nM Compound: 1; (Pyr1)apelin-13	Agonist activity at human APJ-R expressed in HEK293 cells assessed as inhibition of forskolin- stimulated cAMP accumulation incubated for 30 mins by HTRF assay Agonist activity at human APJ-R expressed in HEK293 cells assessed as inhibition of forskolin- stimulated cAMP accumulation incubated for 30 mins by HTRF assay	[PMID: 31724863]
HEK293	EC₅₀	0.062 nM Compound: 1; (Pyr1)apelin-13	Agonist activity at rat APJ-R expressed in HEK293 cells assessed as inhibition of forskolin- stimulated cAMP accumulation incubated for 30 mins by HTRF assay Agonist activity at rat APJ-R expressed in HEK293 cells assessed as inhibition of forskolin- stimulated cAMP accumulation incubated for 30 mins by HTRF assay	[PMID: 31724863]
HEK293	EC₅₀	0.84 nM Compound: Ape13	Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of Galphai1 subunit dissociation incubated for 5 mins by BRET assay Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of Galphai1 subunit dissociation incubated for 5 mins by BRET assay	[PMID: 25668242]
HEK293	EC₅₀	1.1 nM Compound: Ape13	Activity at human APJ receptor expressed in HEK293 cells assessed as dissociation of Galphai1 from Gbetagamma subunit after 5 mins by BRET assay Activity at human APJ receptor expressed in HEK293 cells assessed as dissociation of Galphai1 from Gbetagamma subunit after 5 mins by BRET assay	[PMID: 29461833]
HEK293	IC₅₀	1.2 nM Compound: Ape13	Displacement of [125I]apelin-13[Glp65,Nle75,Tyr77] from YFP epitope-tagged human APJ receptor expressed in HEK293 cell membranes incubated for 1 hr by gamma counting method Displacement of [125I]apelin-13[Glp65,Nle75,Tyr77] from YFP epitope-tagged human APJ receptor expressed in HEK293 cell membranes incubated for 1 hr by gamma counting method	[PMID: 25668242]
HEK293	EC₅₀	1.4 nM Compound: Ape13	Agonist activity at human APJ receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced intracellular cAMP production incubated for 30 mins by TR-FRET assay Agonist activity at human APJ receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced intracellular cAMP production incubated for 30 mins by TR-FRET assay	[PMID: 25668242]
HEK293	EC₅₀	12.2 pM Compound: [pyr1]-apelin-13	Agonist activity at APJ receptor in HEK293 cells assessed as inhibition of forskolin-induced cAMP level incubated for 5 mins prior to forskolin challenge measured after 30 mins by TR-FRET assay Agonist activity at APJ receptor in HEK293 cells assessed as inhibition of forskolin-induced cAMP level incubated for 5 mins prior to forskolin challenge measured after 30 mins by TR-FRET assay	[PMID: 25241924]
HEK293	EC₅₀	24 nM Compound: Ape13	Induction of human HA-tagged APJ receptor internalization expressed in HEK293 cells after 30 mins by ELISA Induction of human HA-tagged APJ receptor internalization expressed in HEK293 cells after 30 mins by ELISA	[PMID: 29461833]
HEK293	EC₅₀	26 nM Compound: Ape13	Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of beta-arrestin-1 recruitment subunit dissociation incubated for 30 mins by BRET assay Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of beta-arrestin-1 recruitment subunit dissociation incubated for 30 mins by BRET assay	[PMID: 25668242]
HEK293	EC₅₀	29 nM Compound: Ape13	Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of beta-arrestin-2 recruitment subunit dissociation incubated for 30 mins by BRET assay Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of beta-arrestin-2 recruitment subunit dissociation incubated for 30 mins by BRET assay	[PMID: 25668242]
HEK293	EC₅₀	5.9 nM Compound: Ape13	Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of GalphaoA subunit dissociation incubated for 5 mins by BRET assay Agonist activity at human APJ receptor expressed in HEK293 cells assessed as induction of GalphaoA subunit dissociation incubated for 5 mins by BRET assay	[PMID: 25668242]
HEK293	EC₅₀	60 nM Compound: Ape13	Activity at GFP10-tagged human APJ receptor expressed in HEK293 cells assessed as induction of RlucII-tagged beta-arrestin-2 recruitment after 30 mins by BRET assay Activity at GFP10-tagged human APJ receptor expressed in HEK293 cells assessed as induction of RlucII-tagged beta-arrestin-2 recruitment after 30 mins by BRET assay	[PMID: 29461833]

体外研究
(In Vitro)

[Pyr1]-apelin-13 包裹在 lipoPEG 颗粒 (lipoPEG-PA13) 中，可在生理条件下实现持续和延长的药物释放^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

[Pyr1]-apelin-13 纳米载体在压力过载引起的心力衰竭小鼠模型中表现出[Pyr1]-apelin-13 在预防心功能障碍方面的可持续长期作用^[1]。[Pyr1]apelin-13 (1，5 μg) 改善大鼠的运动活动并减少疼痛症状、空腔大小和 caspase-3 水平。[Pyr1]apelin-13 (1，5 μg) 显著增加热缩爪潜伏期。与 SCI 后第二周的 SCI 动物相比，5 μg 剂量的[Pyr1]apelin-13 也会显著降低缩爪阈值^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1533.80

Formula

C₆₉H₁₀₈N₂₂O₁₆S

CAS 号

217082-60-5

性状

固体

颜色

White to off-white

Sequence Shortening

{Glp}-RPRLSHKGPMPF

中文名称

[Pyr1]-爱帕琳肽-13

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

细胞实验：

H₂O 中的溶解度 : 100 mg/mL (65.20 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.6520 mL	3.2599 mL	6.5198 mL
5 mM	0.1304 mL	0.6520 mL	1.3040 mL
10 mM	0.0652 mL	0.3260 mL	0.6520 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (65.20 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.92%

选择批次：

Data Sheet (632 KB) SDS (394 KB)

COA (289 KB) LCMS (86 KB) 元素分析报告 (213 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Serpooshan V, et al. [Pyr1]-Apelin-13 delivery via nano-liposomal encapsulation attenuates pressure overload-induced cardiac dysfunction. Biomaterials. 2015 Jan;37:289-98. [Content Brief]

[2]. Hajimashhadi Z, et al. Chronic administration of [Pyr1] apelin-13 attenuates neuropathic pain after compression spinal cord injury in rats. Neuropeptides. 2017 Feb;61:15-22. [Content Brief]

[Pyr1]-Apelin-13 相关分类

Cardiovascular Disease

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.6520 mL	3.2599 mL	6.5198 mL	16.2994 mL
	5 mM	0.1304 mL	0.6520 mL	1.3040 mL	3.2599 mL
	10 mM	0.0652 mL	0.3260 mL	0.6520 mL	1.6299 mL
	15 mM	0.0435 mL	0.2173 mL	0.4347 mL	1.0866 mL
	20 mM	0.0326 mL	0.1630 mL	0.3260 mL	0.8150 mL
	25 mM	0.0261 mL	0.1304 mL	0.2608 mL	0.6520 mL
	30 mM	0.0217 mL	0.1087 mL	0.2173 mL	0.5433 mL
	40 mM	0.0163 mL	0.0815 mL	0.1630 mL	0.4075 mL
	50 mM	0.0130 mL	0.0652 mL	0.1304 mL	0.3260 mL
	60 mM	0.0109 mL	0.0543 mL	0.1087 mL	0.2717 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

[Pyr1]-Apelin-13217082-60-5[pGlu1]-Apelin-13[Pyr1]-爱帕琳肽-13Apelin Receptor (APJ)Inhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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上海工商

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营业执照（三证合一）

[Pyr1]-Apelin-13 (Synonyms: [pGlu1]-Apelin-13)

Customer Review

Other Forms of [Pyr1]-Apelin-13:

MCE 顾客使用本产品发表的 1 篇科研文献