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  4. 3-Indoxyl phosphate sodium

3-Indoxyl phosphate sodium  (Synonyms: 3-IP sodium)

目录号: HY-W009458
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3-Indoxyl phosphate (3-IP) 是碱性磷酸酶的显色和电化学底物。碱性磷酸酶酶促裂解后，3-吲哚酚被释放，随后被氧化，产生不溶于水的靛蓝，可通过 660 nm 的比色检测来量化，作为碱性磷酸酶活性的量度。Sodium 1H-indol-3-yl phosphate (Indol-3-yl sodium phosphate; 3-IP) 还可以通过碱性溶液中的连二亚硫酸钠还原靛蓝转化为水溶性无色靛蓝，可通过 415 nm 的比色检测或伏安检测来量化，作为碱性磷酸酶活性的量度。Sodium 1H-indol-3-yl phosphate (Indol-3-yl sodium phosphate; 3-IP) 已用于 ELISA。

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3-Indoxyl phosphate sodium Chemical Structure

3-Indoxyl phosphate sodium Chemical Structure

CAS No. : 3318-43-2

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生物活性

3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.

分子量

257.09

Formula

C8H6NNa2O4P
CAS 号
中文名称

3-吲羟磷酸二钠盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
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3-Indoxyl phosphate sodium 相关分类

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Keywords:

3-Indoxyl phosphate3318-43-23-IP3-吲羟磷酸二钠盐OthersInhibitorinhibitorinhibit

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