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  2. Fluorescent Dye
  3. 4-Di-1-ASP

4-Di-1-ASP 是一种苯乙烯基染料,可用作活脑组织中的神经胶质瘤细胞染色,用于细胞结构、活力、增殖及内吞、胞吐、吞噬作用分析,也可用于观察活细胞线粒体结构。4-Di-1-ASP 在显微镜成像时可发出绿色荧光 (λexem = 475/606 nm)。

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4-Di-1-ASP Chemical Structure

4-Di-1-ASP Chemical Structure

CAS No. : 959-81-9

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     可免费申领三个不同产品的试用装。

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

4-Di-1-ASP is a styryl dye used to stain glioma cells in living brain tissue for analysis of cell structure, viability, proliferation and endocytosis, cytokinesis and phagocytosis, as well as for observation of mitochondrial structures in living cells. 4-Di-1-ASP fluoresces green when imaged microscopically (λexem = 475/606 nm) [1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1.Dissolve the dye in water and configure a mother liquor at a concentration of 2 mM.
2.Place the prepared cell sections in an incubator at 35°C, add the prepared dye solution to a final concentration of 1 μM and incubate with the stain for 10 min.
3.The prepared stained cell sections are placed on a microscope slide, cover with a coverslip and observe using a fluorescent confocal microscope with a maximum excitation wavelength of 475 nm and a maximum emission wavelength of 606 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

366.24

Formula

C16H19IN2

CAS 号
性状

固体

颜色

Purple to purplish red

Emission (Em)

521

Excitation (Ex)

488

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 43.75 mg/mL (119.46 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7304 mL 13.6523 mL 27.3045 mL
5 mM 0.5461 mL 2.7304 mL 5.4609 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.95%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7305 mL 13.6523 mL 27.3045 mL 68.2613 mL
5 mM 0.5461 mL 2.7305 mL 5.4609 mL 13.6523 mL
10 mM 0.2730 mL 1.3652 mL 2.7305 mL 6.8261 mL
15 mM 0.1820 mL 0.9102 mL 1.8203 mL 4.5508 mL
20 mM 0.1365 mL 0.6826 mL 1.3652 mL 3.4131 mL
25 mM 0.1092 mL 0.5461 mL 1.0922 mL 2.7305 mL
30 mM 0.0910 mL 0.4551 mL 0.9102 mL 2.2754 mL
40 mM 0.0683 mL 0.3413 mL 0.6826 mL 1.7065 mL
50 mM 0.0546 mL 0.2730 mL 0.5461 mL 1.3652 mL
60 mM 0.0455 mL 0.2275 mL 0.4551 mL 1.1377 mL
80 mM 0.0341 mL 0.1707 mL 0.3413 mL 0.8533 mL
100 mM 0.0273 mL 0.1365 mL 0.2730 mL 0.6826 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
4-Di-1-ASP
目录号:
HY-W094758A
需求量: