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  3. 4-Di-10-ASP

4-Di-10-ASP 是一种荧光亲脂性示踪剂 (Excitation 485 nm; Emission 620 nm)。4-Di-10-ASP 可以用来对磷脂膜进行特殊染色。

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4-Di-10-ASP Chemical Structure

4-Di-10-ASP Chemical Structure

CAS No. : 95378-73-7

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5 mg ¥2800
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10 mg ¥4500
In-stock
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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 4-Di-10-ASP (1 μM), DOPC (HY-113424A) (10 mM), and DOPG (HY-142980) (1 mM) is dissolved in methanol/chloroform (25 L, 1:2 v/v).
2. The solution is allowed to dry overnight under vacuum to obtain lamellar lipid films, which in turn are hydrated with the transcription/translation solution (25 L) for three hours at 37℃.
3. An aliquot (10 L) of the solution thus prepared is placed on a glass slide and sealed by a cover glass.
4. The sample is immediately observed with a confocal laser-scanning microscope, an argon laser (488 nm) is employed to excite the 4-Di-10-ASP[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

618.72

Formula

C34H55IN2

CAS 号
性状

固体

颜色

Orange to red

Emission (Em)

607

Excitation (Ex)

485

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 33.33 mg/mL (53.87 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6162 mL 8.0812 mL 16.1624 mL
5 mM 0.3232 mL 1.6162 mL 3.2325 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6162 mL 8.0812 mL 16.1624 mL 40.4060 mL
5 mM 0.3232 mL 1.6162 mL 3.2325 mL 8.0812 mL
10 mM 0.1616 mL 0.8081 mL 1.6162 mL 4.0406 mL
15 mM 0.1077 mL 0.5387 mL 1.0775 mL 2.6937 mL
20 mM 0.0808 mL 0.4041 mL 0.8081 mL 2.0203 mL
25 mM 0.0646 mL 0.3232 mL 0.6465 mL 1.6162 mL
30 mM 0.0539 mL 0.2694 mL 0.5387 mL 1.3469 mL
40 mM 0.0404 mL 0.2020 mL 0.4041 mL 1.0102 mL
50 mM 0.0323 mL 0.1616 mL 0.3232 mL 0.8081 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
4-Di-10-ASP
目录号:
HY-D1630
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