5,5'-Difluoro BAPTA 

5,5'-Difluoro BAPTA is a difluoro-derivative of BAPTA (HY-100168). 5,5'-Difluoro BAPTA is the most widely used probe for studying cytosolic free Ca²⁺ by ¹⁹F NMR. 5,5'-Difluoro BAPTA has high selectivity for Ca²⁺. 5,5'-Difluoro BAPTA can inhibit the growth of pollen tube^[1][2].

5,5'-Difluoro BAPTA shows large ¹⁹F NMR chemical shifts upon chelating divalent cations^[1].
Fe²⁺ ion concentrations were measured following addition of 5 mM 5,5'-Difluoro BAPTA to the culture medium. Fe²⁺ forms a complex with 5,5'-Difluoro BAPTA (Kd=50 nM) that exhibits a characteristic peak down-field from biological ions such as Ca²⁺ and Zn^2+[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

800.66

C₃₄H₃₈F₂N₂O₁₈

156027-00-8

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bar-Shir A, et al. Metal ion sensing using ion chemical exchange saturation transfer 19F magnetic resonance imaging. J Am Chem Soc. 2013 Aug 21;135(33):12164-7.  [Content Brief]

  [2]. Pierson ES, et al. Pollen tube growth is coupled to the extracellular calcium ion flux and the intracellular calcium gradient: effect of BAPTA-type buffers and hypertonic media. Plant Cell. 1994 Dec;6(12):1815-28.  [Content Brief]

  [3]. Kostellow AB, et al. Iron-catalyzed lipid peroxidation in aortic cells in vitro: protective effect of extracellular magnesium. Atherosclerosis. 2004 Jul;175(1):15-22.  [Content Brief]