L162389

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L162389 是一种有效的 angiotensin AT1 受体 拮抗剂，K_i 值为 28 nM。

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L162389 Chemical Structure

CAS No. : 169281-53-2

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AT1 Receptor AT2 Receptor

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

L162389 is a potent antagonist of angiotensin AT1 receptor with K_i of 28 nM.

IC₅₀ & Target

Ki: 28 nM (angiotensin AT1 receptor)^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	1.5 nM Compound: 4; L-162,389	Displacement of 125I-[Sar1,Leu8] angiotensin-2 from rat type 1 angiotensin-2 receptor expressed in African green monkey COS7 cells after 24 hrs Displacement of 125I-[Sar1,Leu8] angiotensin-2 from rat type 1 angiotensin-2 receptor expressed in African green monkey COS7 cells after 24 hrs	[PMID: 26810314]

体外研究
(In Vitro)

L-162,389 stimulates phosphatidylinositol turnover, albeit only to a small percentage of the angiotensin response. L-162,389 acts as angiotensin antagonist with IC₅₀ value of 105 nM^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

562.72

Formula

C₃₁H₃₈N₄O₄S

CAS 号

169281-53-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

产品使用指南 (1538 KB)

参考文献

[1]. Perlman S, et al. Dual agonistic and antagonistic property of nonpeptide angiotensin AT1 ligands: susceptibility to receptor mutations. Mol Pharmacol. 1997 Feb;51(2):301-11. [Content Brief]

Kinase Assay ^[1]	Monoiodinated ¹²⁵I-[Sar1,Leu8]angiotensin II is prepared by the Iodo-Gen method. One day after transfection and 24 hr before the binding experiments, the transfected cells are transferred to 6-, 12-, or 24-well culture plates, with 0.15-9 × 10⁵ cells/well, with a goal of total binding of 5-10% of the radiolabeled peptide. The cells are washed twice with buffer (25 mM Tris, 5 mM MgCl₂, 140 mM NaCl, pH 7.4) before and after the binding. The binding is carried out for 24 hr at 4°C with 50 pm ¹²⁵I-[Sar1,Leu8]angiotensin II and variable amounts of unlabeled nonpeptide or peptide ligands in 0.5-1 mL of a 25 mM Tris buffer containing 5 mm MgCl₂, pH 7.4. The binding data are analyzed by computerized nonlinear regression analysis using InPlot 4.0. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Perlman S, et al. Dual agonistic and antagonistic property of nonpeptide angiotensin AT1 ligands: susceptibility to receptor mutations. Mol Pharmacol. 1997 Feb;51(2):301-11. [Content Brief]

Kinase Assay
^[1]

Monoiodinated ¹²⁵I-[Sar1,Leu8]angiotensin II is prepared by the Iodo-Gen method. One day after transfection and 24 hr before the binding experiments, the transfected cells are transferred to 6-, 12-, or 24-well culture plates, with 0.15-9 × 10⁵ cells/well, with a goal of total binding of 5-10% of the radiolabeled peptide. The cells are washed twice with buffer (25 mM Tris, 5 mM MgCl₂, 140 mM NaCl, pH 7.4) before and after the binding. The binding is carried out for 24 hr at 4°C with 50 pm ¹²⁵I-[Sar1,Leu8]angiotensin II and variable amounts of unlabeled nonpeptide or peptide ligands in 0.5-1 mL of a 25 mM Tris buffer containing 5 mm MgCl₂, pH 7.4. The binding data are analyzed by computerized nonlinear regression analysis using InPlot 4.0.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Perlman S, et al. Dual agonistic and antagonistic property of nonpeptide angiotensin AT1 ligands: susceptibility to receptor mutations. Mol Pharmacol. 1997 Feb;51(2):301-11. [Content Brief]

L162389 相关分类

Cardiovascular Disease

摩尔计算器
稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L162389169281-53-2L 162389L-162389Angiotensin ReceptorInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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沪(浦)应急管危经许[2021]201709(QFYS)

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L162389

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L162389

L162389 相关抗体

L162389 相关产品

•同靶点产品:

•同靶点蛋白产品:

查看 Angiotensin Receptor 亚型特异性产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

L162389 相关抗体:

L162389 相关分类

摩尔计算器

稀释计算器

The molarity calculator equation

The dilution calculator equation

Keywords:

您最近查看的产品:

Request for HNMR Report

Request for HNMR Report

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