2. Stem Cell/Wnt
  3. PKG
  4. PKG Substrate

PKG Substrate 是一种 cGMP 依赖性的蛋白激酶 (PKG) 的选择性底物。

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Custom Peptide Synthesis

PKG Substrate Chemical Structure

PKG Substrate Chemical Structure

CAS No. : 81187-14-6

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规格 价格 是否有货 数量
1 mg ￥1100
In-stock
5 mg ￥3900
In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Customer Review

PKG Substrate 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

PKG Substrate is a selective substrate for cGMP-dependent protein kinase (PKG).

IC50 & Target

PKG[1]
体外研究
(In Vitro)

Incorporation of [33P]ATP into the synthetic peptide PKG substrate RKRSRAE is measured. N6-benzyl-ATP inhibits kinase activity of PKG Iα gatekeeper mutants but not WT. The serotonin transporter (SERT) is responsible for reuptake of serotonin (5-hydroxytryptamine) after its exocytotic release from neurons. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and PKG[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

PKG Substrate 相关抗体:
分子量

902.01

Formula

C35H67N17O11
CAS 号
性状

固体

颜色

White to off-white

Sequence

Arg-Lys-Arg-Ser-Arg-Ala-Glu
Sequence Shortening

RKRSRAE
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据
细胞实验： 

H2O 中的溶解度 : 100 mg/mL (110.86 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1086 mL 5.5432 mL 11.0864 mL
5 mM 0.2217 mL 1.1086 mL 2.2173 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 100 mg/mL (110.86 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Kinase Assay
[1]

Kinase activity is measured by determining the amount of 33P radioactivity incorporated from [33P]ATP or [33P]N6-benzyl-ATP into a PKG specific peptide substrate (RKRSRAE). The standard 75 μL assay mixture contains 0.15 μCi of [33P]ATP, 10 μM ATP, 15 μM PKG peptide substrate, 2 μM PKI (a synthetic peptide inhibitor of cAMP-dependent protein kinase), 1 μg of purified kinase, and 100 μM 8-Br-cGMP in 50 mM HEPES buffer, pH 7.4, containing 10 mM MgCl2, 0.1% Tween 20, and 1 mM DTT. After incubation at 30°C for 2 min, the reaction is immediately put on ice, and 20 μL of the assay mixture is spotted onto P81 phosphocellulose paper and then quenched in 0.42% H3PO4. The paper is further washed three times in 0.42% H3PO4 for 10 min with gentle agitation and rinsed once with acetone. After air drying, radioactivity on the paper is measured with a Beckman LS6500 liquid scintillation counter. For measuring the effect of N6-benzyl-ATP on the activity of PKG I utilizing ATP as a co-substrate, unlabeled N6-benzyl-ATP is added to each reaction at the indicated concentrations. Saturation kinetic analyses for Km and Vmax with ATP or N6-benzyl-ATP are performed over a concentration range (0.0015-100 μM) by adding unlabeled ATP or N6-benzyl-ATP to a given amount of [33P]ATP or [33P]N6-benzyl-ATP, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

PKG Substrate 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1086 mL 5.5432 mL 11.0864 mL 27.7159 mL
5 mM 0.2217 mL 1.1086 mL 2.2173 mL 5.5432 mL
10 mM 0.1109 mL 0.5543 mL 1.1086 mL 2.7716 mL
15 mM 0.0739 mL 0.3695 mL 0.7391 mL 1.8477 mL
20 mM 0.0554 mL 0.2772 mL 0.5543 mL 1.3858 mL
25 mM 0.0443 mL 0.2217 mL 0.4435 mL 1.1086 mL
30 mM 0.0370 mL 0.1848 mL 0.3695 mL 0.9239 mL
40 mM 0.0277 mL 0.1386 mL 0.2772 mL 0.6929 mL
50 mM 0.0222 mL 0.1109 mL 0.2217 mL 0.5543 mL
60 mM 0.0185 mL 0.0924 mL 0.1848 mL 0.4619 mL
80 mM 0.0139 mL 0.0693 mL 0.1386 mL 0.3464 mL
100 mM 0.0111 mL 0.0554 mL 0.1109 mL 0.2772 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PKG Substrate81187-14-6PKGInhibitorinhibitorinhibit

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