Rhod-2 AM

目录号: HY-D0989 纯度: 96.30%: FAQs
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Rhod-2 是一种高亲和可见光激发波长的 Ca²⁺ 荧光探针，Rhod-2 AM 是 Rhod-2 的乙酰甲酯衍生物，具有细胞膜通透性，简单培养即可轻松进入细胞。一旦进入细胞，被其乳糖酶剪切产生无膜渗透性的 Rhod-2，留在细胞内执行相应的生理功能。最大激发/发射波长: 549/578 nm。

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Rhod-2 AM Chemical Structure

CAS No. : 145037-81-6

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规格	价格	是否有货
50 μg	￥600	In-stock
1 mg	￥5800	In-stock
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MCE 顾客使用本产品发表的 15 篇科研文献

: Rhod-2 AM purchased from MCE. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948. [Abstract]; Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa₂O₃ treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.

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生物活性
实验参考方法
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参考文献

生物活性	Rhod-2 is a high-affinity visible light excitation wavelength Ca²⁺ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm^[1].
体外研究 (In Vitro)	1. Rhod-2 AM工作液的配制 1.1 原液的制备将 Rhod-2 AM 溶解在 DMSO 中，获得 5 mM 储备溶液。 1.2 Rhod-2 AM工作液的配制用无血清细胞培养基或 PBS 稀释储备液，以获得 5-10 μM 的工作溶液。注：请根据实际情况调整 Rhod-2 AM 工作液的浓度。 2. 细胞染色（6孔板） 2.1 悬浮细胞 a. 4℃、1000g 离心 3-5 分钟，弃上清。PBS 洗两次，每次 5 分钟。细胞密度为 1×10⁶/mL。 b.加入1 mL 工作液，然后室温孵育 5-30 分钟。 c. 4℃、400g 离心 3-4 分钟，弃上清。 d. 用 PBS 洗涤两次，每次 5 分钟。 e. 用无血清细胞培养基或 PBS 重悬细胞。通过荧光显微镜或流式细胞术观察。 2.2 贴壁细胞 a. 在无菌盖玻片上培养贴壁细胞。 b. 从培养基中取出盖玻片并吸出多余的培养基。 c. 加入100 μL 工作液，轻轻摇匀，使细胞完全覆盖，室温孵育 5-30 分钟。 d. 用培养基洗涤两次，每次 5 分钟。荧光显微镜或流式细胞仪观察。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. Rhod-2 AM 相关抗体:
分子量	1123.94
Formula	C₅₂H₅₉BrN₄O₁₉
CAS 号	145037-81-6
性状	油状物
颜色	Dark green to black
Emission (Em)	578
Excitation (Ex)	549
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
纯度 & 产品资料	纯度: ≥97.0% 选择批次： Data Sheet (563 KB) SDS (252 KB) COA (296 KB) 产品使用指南 (1538 KB)
染色示例	Description: Rhod-2 AM is a mitochondrial probe, it can be used to detect mitochondrial Ca²⁺ levels with red fluorescence. Method: For mitochondrial potential assessment. 1. After treatment, cells are incubated at 37°C in dark with 4 µM Rhod-2 AM for 20 min. 2. Wash cells for three times and re-suspended in Hank’s Balanced Salt Solution without Ca²⁺ and Mg²⁺. 3. Treat cells with REE NPs and additionally stained with 20 nM TMRM, then analysed by flow cytometry (FACS) for mitochondrial membrane assessment. FACS analysis is performed using an LSR II flow cytometer (Becton Dickinson, Mountain View, CA) equipped with a single 488 nm argon laser. Rhod-2 fluorescence are examined using the PE channel; and both forward and side scatters are used to eliminate cellular fragments. 4. Following treatment, a portion of cells are also fixed with 10% PBS-buffered formaldehyde with 0.1% Triton X-100 for 10 min, then stained with 4’ 6-diamidino-2-phenylindole (DAPI) (blue) and Fluo-3/AM (green) or Rhod-2 (red). Fluorescent images are visualized through confocal laser scanning microscopy. Description: Rhod-2 AM is a mitochondrial probe, it can be used to detect mitochondrial Ca²⁺ levels with red fluorescence. Method: For measurement of mitochondrial Ca²⁺ levels. 1. Cells are incubated with Rhod-2 AM (10 μM; 2 h; dark). 2. Wash cells twice with PBS, and then resuspended in PBS. 3. Fluorescence is determined by flow cytometry, and fluorescence photomicrographs are taken using a fluorescence microscope. Description: Rhod-2 AM is a Ca²⁺ fluorescence probe that specifically aggregates in mitochondria because of its positive charge, it can be used to track mitochondrial Ca²⁺ uptake in cells. Method: For measurement of mitochondrial Ca²⁺ uptake in oocytes. 1. Around 40 oocytes are incubated with Rhod-2 AM (6 µM) in the KSOM medium for 30 min at 37°C. 2. After three quick washes with the KSOM medium, the oocytes are imaged using a Nikon fluorescence microscope (Nikon, Tokyo, Japan).
参考文献	[1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [Content Brief] [2]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [Content Brief]

Cell Assay ^[1]	For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×10⁵ cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [Content Brief] [2]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [Content Brief]

Cell Assay
^[1]

For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×10⁵ cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [Content Brief]

[2]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [Content Brief]

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Rhod-2 AM

MCE 顾客使用本产品发表的 15 篇科研文献

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Rhod-2 AM

Rhod-2 AM 相关抗体

MCE 顾客使用本产品发表的 15 篇科研文献

Rhod-2 AM 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

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参考文献

Rhod-2 AM 相关抗体:

Rhod-2 AM 相关分类

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