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  3. Antisenescence Expansion of Mesenchymal Stem Cells Using Piezoelectric β-Poly(vinylidene fluoride) Film-Based Culture

Antisenescence Expansion of Mesenchymal Stem Cells Using Piezoelectric β-Poly(vinylidene fluoride) Film-Based Culture

  • ACS Appl Mater Interfaces. 2024 Nov 20;16(46):63207-63224. doi: 10.1021/acsami.4c12725.
Liuyue Xu 1,  Wenxiang Ren 2,  Yaoying Long 3,  Bianlei Yang 1,  Li Chen 4,  Wenlan Chen 3,  Siyi Chen 1,  Yulin Cao 1,  Di Wu 3,  Jiao Qu 3,  He Li 3,  Yali Yu 1,  Anyuan Zhang 3,  Shan Wang 1,  Hongxiang Wang 4,  Ting Chen 5,  Guifen Fan 6 7,  Qiubai Li 1 5,  Zhichao Chen 3
Affiliations

Affiliations

  • 1 Department of Rheumatology and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 2 Center for Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
  • 3 Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 4 Department of Hematology, Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.
  • 5 Hubei Engineering Research Center for Application of Extracellular Vesicles, Hubei University of Science and Technology, Xianning 437100, China.
  • 6 School of Optical and Electronic Information, Key Lab of Functional Materials for Electronic Information(B), MOE, Huazhong University of Science and Technology, Wuhan 430074, China.
  • 7 Wenzhou Advanced Manufacturing Institute, Huazhong University of Science and Technology, Wenzhou 325035, China.
PMID: 39503875 DOI: 10.1021/acsami.4c12725
Abstract

Regenerative therapies based on mesenchymal stem cells (MSCs) show promise in treating a wide range of disorders. However, the replicative senescence of MSCs during in vitro expansion poses a challenge to obtaining a substantial quantity of high-quality MSCs. In this investigation, a piezoelectric β-poly(vinylidene fluoride) film-based culture plate (β-CP) was developed with an antisenescence effect on cultured human umbilical cord-derived MSCs. Compared to traditional tissue culture plates (TCPs) and α-poly(vinylidene fluoride) film-based culture plates, the senescence markers of p21, p53, interleukin-6 and insulin-like growth factor-binding protein-7, stemness markers of OCT4 and NANOG, and telomere length of MSCs cultured on β-CPs were significantly improved. Additionally, MSCs at passage 18 cultured on β-CPs showed significantly better multipotency and pro-angiogenic capacities in vitro, and higher wound healing abilities in a mouse model. Mechanistically, β-CPs rejuvenated senescent MSCs by improving mitochondrial functions and mitigating oxidative and glycoxidative stresses. Overall, this study presents β-CPs as a promising approach for efficient and straightforward antisenescence expansion of MSCs while preserving their stemness, thereby holding great potential for large-scale production of MSCs for clinical application in cell therapies.

Keywords

antisenescence; cell therapy; large-scale production; mesenchymal stem cells; piezoelectric PVDF films; stemness.

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