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  3. Fluorescent Dye
  4. Acrylodan

Acrylodan 可与硫醇反应，对 Cys34 周围的结合口袋内的局部环境双极性和动力学敏感。

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Acrylodan Chemical Structure

Acrylodan Chemical Structure

CAS No. : 86636-92-2

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规格 价格 是否有货 数量
5 mg ￥800
In-stock
10 mg ￥1240
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

Acrylodan 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M 1 cm 1 for actin at 290 nm, 18,500 M 1 cm 1 or acrylodan at 385 nm, and 71,000 M 1 cm 1 for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (AcrylAct1NAT) is dramatically different than that of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Acrylodan 相关抗体:
分子量

225.29

Formula

C15H15NO
CAS 号
性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 50 mg/mL (221.94 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.4387 mL 22.1936 mL 44.3872 mL
5 mM 0.8877 mL 4.4387 mL 8.8774 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 95.05%

COA (283 KB) RP-HPLC (205 KB)

产品使用指南 (1538 KB)
参考文献

Acrylodan 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4387 mL 22.1936 mL 44.3872 mL 110.9681 mL
5 mM 0.8877 mL 4.4387 mL 8.8774 mL 22.1936 mL
10 mM 0.4439 mL 2.2194 mL 4.4387 mL 11.0968 mL
15 mM 0.2959 mL 1.4796 mL 2.9591 mL 7.3979 mL
20 mM 0.2219 mL 1.1097 mL 2.2194 mL 5.5484 mL
25 mM 0.1775 mL 0.8877 mL 1.7755 mL 4.4387 mL
30 mM 0.1480 mL 0.7398 mL 1.4796 mL 3.6989 mL
40 mM 0.1110 mL 0.5548 mL 1.1097 mL 2.7742 mL
50 mM 0.0888 mL 0.4439 mL 0.8877 mL 2.2194 mL
60 mM 0.0740 mL 0.3699 mL 0.7398 mL 1.8495 mL
80 mM 0.0555 mL 0.2774 mL 0.5548 mL 1.3871 mL
100 mM 0.0444 mL 0.2219 mL 0.4439 mL 1.1097 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Acrylodan86636-92-2Fluorescent DyeChaperoneChaperoninProteinFoldingAMP-PNPPhosducin-likeInhibitorinhibitorinhibit

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