M-CSF 抗体

M-CSF Antibody is a non-conjugated and Rabbit origined monoclonal antibody, targeting to M-CSF. It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human.

M-CSF：该基因编码的蛋白质是一种细胞因子，可控制巨噬细胞的产生、分化和功能。该蛋白质的活性形式在细胞外被发现为二硫键连接的同源二聚体，并且被认为是由膜结合前体的蛋白水解切割产生的。编码的蛋白质可能参与胎盘的发育。交替剪接导致多个转录本变体。[由 RefSeq 提供，2011 年 9 月]

Free

1435

Predicted band size: 43 kDa

affinity purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P09603

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-F, IHC-P, ICC/IF

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human M-CSF.The exact sequence is proprietary to MCE.