Phospho-Glycogen synthase 1 (S641) 抗体

Phospho-Glycogen synthase 1 (S641) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 84 kDa, targeting to Phospho-Glycogen synthase 1(S641). It can be used for WB,ICC,IHC-P,IP assays with tag free, in the background of Human, Mouse, Rat.

Free

Predicted band size: 84 kDa

Protein A affinity purified.

Cytoplasm

Non-conjugated

Phosphorylated

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

SwissProt: P13807 Human;SwissProt: Q9Z1E4 Mouse;Entrez Gene: 690987 Rat

WB: 1:500 ;ICC: 1:50 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from HepG2 (lane 2(20μg) or lane 3(40μg)), using Phospho-Glycogen synthase 1(HY-P80277) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80277, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser641 of Human Glycogen synthase 1.The exact sequence is proprietary to MCE.