Phospho-Smad1 (Ser463/Ser465) 抗体

Phospho-Smad1 (Ser463/Ser465) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 52 kDa, targeting to Phospho-Smad1 (Ser463/Ser465). It can be used for WB,IP assays with tag free, in the background of Human, Rat.

Smad1：由该基因编码的蛋白质属于 SMAD，这是一个蛋白质家族，类似于果蝇基因“抗脑瘫母亲”(Mad) 和秀丽隐杆线虫基因 Sma 的基因产物。SMAD 蛋白是介导多种信号通路的信号转导和转录调节剂。这种蛋白质介导骨形态发生蛋白 (BMP) 的信号，骨形态发生蛋白参与一系列生物活动，包括细胞生长、细胞凋亡、形态发生、发育和免疫反应。响应于 BMP 配体，该蛋白可被 BMP 受体激酶磷酸化和激活。这种蛋白质的磷酸化形式与 SMAD4 形成复合物，这对其在转录调节中的功能很重要。该蛋白是 SMAD 特异性 E3 泛素连接酶（如 SMURF1 和 SMURF2）的靶标，并经历泛素化和蛋白酶体介导的降解。已经观察到编码相同蛋白质的可变剪接转录物变体。[由 RefSeq 提供，2008 年 7 月]

Free

4086

Predicted band size: 52 kDa

affinity purified

Non-conjugated

Phosphorylated

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

Q15797

WB: 1:500-1:1,000 ;IP: 1:20

• 
  Western blot analysis of extracts from A549(lane 2(20μg) or lane 3(40μg)) and C6(lane 4(20μg) or lane 5(40μg)), using Phospho-Smad1 (HY-P80852) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80852, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser463/Ser465 of Human Smad1.The exact sequence is proprietary to MCE.