PKA R2 抗体

PKA R2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 46 kDa., targeting to PKA R2. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human.

Free

Predicted band size: 46 kDa;Observed band size: 55 kDa

Protein A affinity purified.

Cell membrane, Cytoplasm, Membrane.

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

SwissProt: P13861 Human

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;IP: 1:10-1:50

• 
  Western blot analysis of extracts from K562(lane 2(40μg) ) ,Hela(lane 3(40μg) )and Jurkat(lane 4(40μg) ) using PKA R2 (HY-P80429) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80429, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PKA R2.AA range:272-404.