VASP 抗体

VASP Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 46 kDa, targeting to VASP. It can be used for WB,IP assays with tag free, in the background of Human.

Free

Predicted band size: 46 kDa

Protein A affinity purified.

Cytoplasm, Cell junction, Cell projection.

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

SwissProt: P50552 Human

WB: 1:1,000-1:2,000

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and MCF-7(lane 4(20μg) or lane 5(40μg)), using VASP (HY-P80368) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80368, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human VASP.AA range:51-100.