2. Autophagy Apoptosis
  3. Autophagy Apoptosis
  4. Autophagy-IN-2

Autophagy-IN-2 (Compound 7h) 是一种自噬通量 (autophagic flux) 抑制剂。Autophagy-IN-2 诱导癌细胞凋亡 (apoptosis)，可用于三阴性乳腺癌研究。

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Autophagy-IN-2 Chemical Structure

Autophagy-IN-2 Chemical Structure

CAS No. : 2755454-90-9

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Autophagy-IN-2 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Autophagy-IN-2 (Compound 7h) is an autophagic flux inhibitor. Autophagy-IN-2 induces cancer cell apoptosis and can be used for triple-negative breast cancer research[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
ASPC1 IC50
26.89 μM
Compound: 7h
Antiproliferative activity against human ASPC cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human ASPC cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
HCT-116 IC50
35.53 μM
Compound: 7h
Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
HSC-3 IC50
24.03 μM
Compound: 7h
Antiproliferative activity against human HSC-3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human HSC-3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
LN-229 IC50
61.35 μM
Compound: 7h
Antiproliferative activity against human LN-229 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human LN-229 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
MDA-MB-231 IC50
8.3 μM
Compound: 7h
Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
MDA-MB-468 IC50
6.03 μM
Compound: 7h
Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
PANC-1 IC50
19.25 μM
Compound: 7h
Antiproliferative activity against human PANC cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human PANC cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
PC-3 IC50
17.48 μM
Compound: 7h
Antiproliferative activity against human PC-3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human PC-3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
SCC-25 IC50
18.64 μM
Compound: 7h
Antiproliferative activity against human SCC-25 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human SCC-25 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
SW-620 IC50
25.43 μM
Compound: 7h
Antiproliferative activity against human SW-620 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human SW-620 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
U-251 IC50
12.92 μM
Compound: 7h
Antiproliferative activity against human U-251 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human U-251 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
U-87MG ATCC IC50
15.37 μM
Compound: 7h
Antiproliferative activity against human U87 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human U87 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 35797901]
体外研究
(In Vitro)

Autophagy-IN-2 (Compound 7h) (0-200 μM, 48 h) shows anti-viability activities against various cancer cells[1].
Autophagy-IN-2 (0-20 μM, 0-48 h) suppresses autophagic flux and impairs DNA repair through down-regulating chromatin ubiquitination in a p62-dependent manner[1].
Autophagy-IN-2 (0-20 μM, 48 h) induces cell cycle arrest at S-phase and mitochondria-dependent intrinsic apoptosis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Autophagy-IN-2 相关抗体:

Cell Viability Assay[1]

Cell Line: MDA-MB-231, MDA-MB-468, SCC25, HSC-3, HCT116, SW620, PC3, Aspc, PNAC, U87, U251 and LN229
Concentration: 0-200 μM
Incubation Time: 48 h
Result: Showed anti-viability activities with IC50 values of 8.31 ± 1.05, 6.03 ± 0.82, 18.64 ± 2.92, 24.03 ± 3.75, 35.53 ± 7.52, 25.43 ± 2.42, 17.48 ± 1.27, 26.89 ± 5.23, 19.25 ± 3.96, 15.37 ± 1.78, 12.92 ± 2.38 and 61.35 ± 11.61 μM against MDA-MB-231, MDA-MB-468, SCC25, HSC-3, HCT116, SW620, PC3, Aspc, PNAC, U87, U251 and LN229 cells, respectively.

Western Blot Analysis[1]

Cell Line: MDA-MB-231 and MDA-MB-468
Concentration: 5, 10 and 20 μM
Incubation Time: 0, 6, 12, 24, 36 and 48 h
Result: Significantly induced to LC3B-II in a dose - and time-dependent manner, resulted in a p62 accumulation in TNBC cells, increased γH2AX in a dose and time-dependent manner, activated the phosphorylation of ATM, NBS1 and SMC1, increased p62 in the nucleus and cytoplasm in a dose-dependent manner, and significantly reduced DNA-damage-induced formation of H2A poly-ubiquitin chains.
Decreased the cellular levels of Cyclin A, Cyclin B, CDK1 and CDK2, increased P21 and the phosphorylation levels of CHK1 (Serine 345) and CHK2 (Threonine 68), and increased cytochrome c, cleaved caspase-3, cleaved caspase-9 and cleaved PAPR in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: MDA-MB-231 and MDA-MB-468
Concentration: 5, 10 and 20 μM
Incubation Time: 48 h
Result: Induced cell cycle arrest at S-phase.

Apoptosis Analysis[1]

Cell Line: MDA-MB-231 and MDA-MB-468
Concentration: 5, 10 and 20 μM
Incubation Time: 48 h
Result: Dramatically induced cell apoptosis in TNBC cells and obviously increase the proportion of late-phase apoptosis in a dose-dependent manner.
体内研究
(In Vivo)

Autophagy-IN-2 (Compound 7h) (5 and 15 mg/kg; i.p.; every 3 days for 3 weeks) suppresses tumor growth in a dose-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week-old female NOD/SCID mice, MDA-MB-231 xenograft[1]
Dosage: 5 mg/kg, 15 mg/kg
Administration: Intraperitoneal injection, every 3 days for 3 weeks
Result: Suppressed human TNBC tumor growth in a dose-dependent manner and resulted in a concentration-dependent upregulation of LC3B-II, p62, γH2AX and PARP.
分子量

309.37

Formula

C17H19N5O
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Autophagy-IN-2 相关分类

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  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Autophagy-IN-22755454-90-9AutophagyApoptosistriple-negative breast cancerTNBCMDA-MB-231MDA-MB468Inhibitorinhibitorinhibit

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