1. Biology Dictionary
  2. Beta Galactosidase Complementation

Beta Galactosidase Complementationβ-半乳糖苷酶互补法

释义:

Beta-galactosidase activity can be used to monitor the interaction of chimeric proteins. Pairs of inactive beta gal deletion mutants are capable of complementing to restore activity when fused to interacting protein partners. Critical to the success of this system is the choice of two poorly complementing mutant moieties, since strongly complementing mutants spontaneously assemble and produce functional beta-gal activity detectable in absence of any fused protein fragment.

β-半乳糖苷酶活性可以用来监测嵌合蛋白之间的相互作用。无活性的 β-半乳糖苷酶缺失突变体在与相互作用的蛋白质伙伴融合时能够恢复活性。该系统成功的关键在于选择两种互补性差的突变体,因为高度互补的突变体会自发组装并产生功能性 β-半乳糖苷酶活性,而无需任何融合的蛋白片段。

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