1. Biology Dictionary
  2. Chromatin Immunoprecipitation Assay

Chromatin Immunoprecipitation Assay染色质免疫沉淀测定法

释义:

Chromatin immunoprecipitation (ChIP) is a powerful approach that allows one to define the interaction of factors with specific chromosomal sites in living cells. An antibody against a protein suspected of binding a given cis-element is used to immunoprecipitate fragmented chromatin fragments. Cells or tissue may first be briefly treated with an agent such formaldehyde to crosslink proteins to DNA. Nucleic acids are then identified by sequencing, for example polymerase chain reaction analysis of the immunoprecipitate with primers flanking the cis-element or next-generation sequencing techniques

染色质免疫共沉淀 (ChIP) 是一种强有力的方法,可以定义因子与活细胞中特定染色体位点的相互作用。使用针对怀疑结合某一顺式元件的蛋白质的抗体,免疫共沉淀裂解的染色质片段。细胞或组织可以首先用福尔马林等试剂进行短时间处理,以交联蛋白质与 DNA。然后,通过测序 (例如,使用引物扩增顺式元件两侧的免疫共沉淀产物或使用下一代测序技术) 来鉴定核酸。

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