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  3. Cosedimentation Through Density Gradient

Cosedimentation Through Density Gradient基于密度梯度分离的共沉降

释义：

Sedimentation through a density gradient measures the sedimentation rate of a mixture of proteins through either a glycerol or sucrose gradient. Two interacting proteins will sediment mostly as a complex at concentrations above the binding constant. By varying the concentration of one or both of the complex constituents and taking into account the dilution of the species during sedimentation, one can reasonably accurately estimate the binding constant.

通过密度梯度沉降测量一组蛋白质在甘油或蔗糖梯度中的沉降速率。两个相互作用的蛋白质在浓度超过结合常数时大多作为复合物沉降。通过改变一个或两个复合物组分的浓度，并考虑沉降过程中物种的稀释，可以合理准确地估算结合常数。

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