1. Biology Dictionary
  2. Electron Microscopy

Electron Microscopy电子显微镜

释义:

Electron microscopy methods provide insights into the structure of biological macromolecules and their supramolecular assemblies. Resolution is on average around 10 Angstroms but can reach the atomic level when the samples analysed are 2D crystals. Different types of samples can be analysed by electron microscopy: crystals, single particles like viruses, macromolecular complexes or entire cells and tissue sections. Samples can be chemically fixed or vitrified by rapid freezing in liquid ethane, and then transferred into the electron microscope. Data collection consists of the recording of electron diffraction data (2D crystals) and images. Depending on the type of sample, different approaches are used to analyse and merge images and electron diffraction data.

电子显微镜方法提供了关于生物大分子及其超分子组装结构的见解。分辨率通常在 10 埃左右,但当分析的样本是二维晶体时,分辨率可以达到原子级别。不同类型的样本可以通过电子显微镜进行分析:晶体、单个粒子如病毒、大分子复合物或整个细胞和组织切片。样本可以通过化学固定或快速冷冻在液态乙烷中进行玻璃化,然后转移到电子显微镜中。数据收集包括记录电子衍射数据 (二维晶体) 和图像。根据样本的类型,使用不同的方法来分析和合并图像和电子衍射数据。

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