1. Biology Dictionary
  2. Ion Exchange Chromatography

Ion Exchange Chromatography离子交换色谱法

释义:

Ion Exchange Chromatography is a separation technique based on the differences in net charge of molecules. Proteins or other charged biomolecules are adsorbed onto a chromatographic medium with an opposite charge based on their net charge at a specific pH. By altering the ion strength or pH of the elution buffer, the molecules adsorbed on the column can be gradually eluted. This method is widely used in protein purification, complex separation, and the charge analysis of biomolecules.

离子交换色谱法是一种基于分子净电荷差异的分离技术。蛋白质或其他带电生物分子根据其在特定pH条件下的净电荷被吸附到相反电荷的色谱介质上。通过改变洗脱液中的离子强度或pH值,可以逐步洗脱吸附在色谱柱上的分子。这种方法广泛应用于蛋白质纯化、复合物分离和生物分子的电荷分析。

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