2. Biology Dictionary
  3. Protein Cross-Linking with a Bifunctional Reagent

Protein Cross-Linking with a Bifunctional Reagent使用双功能试剂进行蛋白质交联

释义：

Protein Cross-Linking with a Bifunctional Reagent induces covalent bond formation between adjacent proteins using crosslinkers. The crosslinkers are bifunctional molecules with two reactive ends connected by spacers, typically including disulfide bonds. When a reducing agent is added, the disulfide bonds are cleaved, and the crosslinked pairs are released and can be identified. There are various types of crosslinkers, with the most common being those with photo-reactive groups that become reactive fluorescent probes when exposed to UV light, marking the crosslinked molecules. This technique is widely used to study the spatial relationships, interaction stability, and distance between molecules in protein complexes.

此方法通过交联剂诱导相邻蛋白质之间形成共价键。交联剂可能是具有两个反应端的双功能分子，由间隔物链接，通常包含二硫键。当加入还原剂时，二硫键被切断，交联的对会被释放出来并可被识别。有多种类型的交联剂，其中最常见的是那些具有光反应性基团的交联剂，当受到紫外光照射时，这些基团会变成反应性荧光探针，从而使交联的分子标记。此技术广泛用于研究蛋白质复合物的空间关系、相互作用稳定性以及相邻分子之间的距离。

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