1. Biology Dictionary
  2. Sandwich Immunoassay

Sandwich Immunoassay三明治免疫分析法

释义:

Sandwich Immunoassay uses an array of antibodies to capture the target protein and is identified through detecting antibodies. Detection antibodies can be directly labeled with detectable molecules (such as enzymes, fluorescent molecules, or isotopes), or labeled by biotinylation and binding to streptavidin, widely used for protein quantification, phosphorylation state detection, and biomarker analysis.

三明治免疫分析法利用阵列抗体固定靶蛋白,并通过检测抗体进行识别。检测抗体可直接标记为可检测分子 (如酶、荧光分子或同位素), 或通过生物素化与链霉亲和素标记结合,广泛用于蛋白质定量、磷酸化状态检测和生物标志物分析。

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